关键词: 重金属耐受菌, 肠杆菌属, 16S rDNA, 最低抑菌浓度（MIC）, 去除率

Abstract: A strain that resist to heavy metals with high concentration，named ZM-12, was isolated by using selective culture medium containing different concentrations of heavy metals. It was identified as Enterobacter according to the BLAST comparison, the analysis in MEGA 4.0 and the phylogenetic tree. ZM-12 was Gram-negative by using the Gram stain test. In different heavy metal medium, the MIC value is different. It can be tolerant of Cu2+ 22.33 mmol/L, Ni2+ 58.69 mmol/L, Pb2+ 17.84 mmol/L and Mn2+>200mmol/L. The removal rate of heavy metals can be obtained by atomic absorption spectroscopy (AAS). The removal rate of Pb2+, Mn2+, Cu2+, Ni2+ were 97.68%, 99.93%, 41.23%, 99.46%, respectively. We can conclude that ZM-12 has high resistance to many different heavy metals. The isolation and identification of this strain can provide some basis for the pollution caused by heavy metals.

Key words: strain with heavy metals tolerance, Enterobacter, 16S rDNA, minimum inhibitory concentration (MIC), the removal rate