生物技术进展 ›› 2015, Vol. 5 ›› Issue (5): 382-386.DOI: 10.3969/j.issn.2095-2341.2015.05.10

• 研究论文 • 上一篇    下一篇

食线虫真菌圆锥掘氏梅里霉的高效培养基研究

郭倩楠,周正富,徐玉泉,张维*   

  1. 中国农业科学院生物技术研究所, 北京 100081
  • 收稿日期:2015-05-18 出版日期:2015-09-25 发布日期:2015-06-16
  • 通讯作者: 张维,研究员,主要从事环境微生物分子生物学研究。E-mail:zhangwei01@caas.cn
  • 作者简介:郭倩楠,硕士研究生,主要从事环境微生物基因工程研究。E-mail:guoqiannan0709@163.com。
  • 基金资助:

    国家973计划项目(2015CB755703);国家转基因生物新品种培育重大专项(2014ZX0800301B)资助。

Study on an Efficient Medium to Culture the Nematophagous Fungus Drechmeria coniospora

GUO Qian-nan, ZHOU Zheng-fu, XU Yu-quan, ZHANG Wei   

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-05-18 Online:2015-09-25 Published:2015-06-16

摘要: 作为一种内寄生食线虫真菌,圆锥掘氏梅里霉(Drechmeria coniospora)在常规真菌培养基(如PDA、SDAY和CMA)中生长周期较长(最短为13 d),不利于对其进行科学研究。应用LK-100固体培养基常温培养D. coniospora,通过显微镜观察其生长状态,确定生长周期。研究表明,D. coniospora在LK-100培养基中的生长周期仅8 d,远短于在常规培养基上的生长周期。此外,与常规培养基相比,该培养基获得的D. coniospora分生孢子具有更强的侵染宿主秀丽隐杆线虫(Caenorhabditis elegans)的能力。LK-100培养基为进行D. coniospora相关研究提供了快速、高效的培养方法。

关键词: 圆锥掘氏梅里霉, LK培养基, 生长周期, 侵染能力

Abstract: There is an obstacle to doing scientific research on nematophagous fungus Drechmeria coniospora, owing to the shortest growth cycle of the microbe in conventional medium (including PDA, SDAY and CMA) is 13 days at least.  In this study, we utilized LK-100 medium to culture D. coniospora at room temperature, and observed its growth status under microscope. The results showed that growth cycle of D. coniospora on LK-100 medium was only 8 days. Furthermore, the infection efficiency of conidia, obtained from LK-100 medium, to natural host Caenorhabditis elegans was higher than that obtained from conventional fungi media. LK-100 medium, a kind of effective medium, will contribute to the further study of D. coniospora.