生物技术进展 ›› 2012, Vol. 2 ›› Issue (5): 349-353.DOI: 10.3969/j.issn.2095-2341.2012.05.08

• 研究论文 • 上一篇    下一篇

一株产蓝色素链霉菌18-A-5的鉴定与系统发育分析

李良秋,羊宋贞,朱红惠*   

  1. 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地, 广州 510070
  • 收稿日期:2012-06-29 出版日期:2012-09-25 发布日期:2012-08-12
  • 通讯作者: 朱红惠,研究员,主要从事微生物资源环境方向研究。Tel:020-87684631;E-mail:zhuhonghui66@yahoo.com.cn
  • 作者简介:李良秋,高级工程师,研究方向为微生物资源与发酵工程。

Identification and Phylogenetic Analysis of a Streptomycete Strain 18-A-5 with Blue Diffusible Pigment

LI Liang-qiu, YANG Song-zhen, ZHU Hong-hui   

  1. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology(MinistryGuangdong Province Jointly Breeding Base), South China, Guangzhou, Guangdong 510070, China
  • Received:2012-06-29 Online:2012-09-25 Published:2012-08-12

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摘要： 从原始热带雨林土壤中,分离到一株产蓝色色素菌株18-A-5,对其进行了系统分类学研究。形态学特征观察表明,在高氏合成一号培养基上初产蓝绿色色素,日久为深蓝色,基内菌丝蓝色,气生菌丝灰白色,产灰色孢子,孢子丝直或柔曲,形成长孢子链,孢子圆柱形。其DNA的G＋C摩尔分数为62.4%,16S rDNA序列分析结果(GenBank登陆号为EU054353),18-A-5与生靛链霉菌Streptomyces indigoferus ATCC23924T 、草绿色链霉菌Streptomyces herbaricolor ATCC23924T具有极高的同源性,达100％,聚类分析表明,18-A-5与生靛链霉菌Streptomyces indigoferus、草绿色链霉菌Streptomyces herbaricolor两株菌聚类在一起,分支置信度为74%。结合生理生化特性、细胞壁化学组成分析、脂肪酸分析等将菌株18-A-5定名为草绿色链霉菌Streptomyces herbaricolor。并对该蓝绿色可溶性色素性质进行了耐酸碱性、热稳定性、抗菌谱等初步分析。

关键词: 蓝色素, 链霉菌, 16S rDNA, 系统发育

Abstract: A strain 18-A-5 produced blue diffusible pigment was isolated from a tropical rainforest soil sample. Morphological observation showed that on Gause's synthetic agar medium the strain produced large quantities of blue diffusible pigment, blue substrate mycelium, grey-white aerial mycelium, grey spore, long spore chains straight or flexuous, spores were cylindrical and smooth. The genomic DNA G+C content of the strain was 62.4%. Analysis of the 16S rDNA gene sequence(GenBank accession no. EU054353) of strain 18-A-5 revealed that the highest similarity (100%) was to Streptomyces indigoferus ATCC23924T and Streptomyces herbaricolor ATCC23924T. Phylogenetic tree of Streptomyces 16S rDNA gene sequences showed that 18-A-5 strain was clustered with Streptomyces indigoferus and Streptomyces herbaricolor, the confidence branch was 74%.On the basis of its physiological and molecular properties, it is evident that the strain 18-A-5 belongs to Streptomyces herbaricolor. The physicochemical characterization of pigment was studied.

Key words: blue pigment, streptomycete, 16S rDNA, phylogenesis

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