微波诱变结合抗性突变筛选放线菌素D高产菌株

doi:10.3969/j.issn.2095-2341.2015.05.09

生物技术进展 ›› 2015, Vol. 5 ›› Issue (5): 377-381.DOI: 10.3969/j.issn.2095-2341.2015.05.09

微波诱变结合抗性突变筛选放线菌素D高产菌株

任林英,张祝兰*,王徳森,杨煌建,唐文力,孙菲

福建省微生物研究所, 福建省新药(微生物)筛选重点实验室, 福州 350007

收稿日期:2015-05-08 出版日期:2015-09-25 发布日期:2015-06-15
通讯作者: 张祝兰，研究员，研究方向为微生物药物。E-mail:jessylan9963@sina.com
作者简介:任林英，高级工程师，研究方向为微生物药物。E-mail:rlymary@aliyun.com。
基金资助:
福建省科技项目(2014R1009-10) 资助。

Screening of Actinomycin D High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis

REN Lin-ying, ZHANG Zhu-lan, WANG De-sen, YANG Huang-jian, TANG Wen-li, SUN Fei

Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, China

Received:2015-05-08 Online:2015-09-25 Published:2015-06-15

摘要/Abstract

摘要： 采用微波结合链霉素抗性筛选法选育放线菌素D的高产菌株。通过考察链霉素对Streptomyces rubiginosohelvolus FIM-N31菌株孢子生长情况的影响确定链霉素致死浓度，出发菌株FIM-N31的孢子经微波辐照处理后，涂布在含链霉素致死浓度（50 μg/mL）的培养基平板上培养，获得了大量的链霉素抗性基因突变株。摇瓶发酵筛选突变株，结果获得一株遗传性状稳定的放线菌素D高产菌Str186，其产放线菌素D的能力比出发菌株提高了8倍以上。

关键词: 链霉菌, 放线菌素 D, 抗生素抗性筛选, 复合诱变

Abstract: Microwave mutagenesis and streptomycin resistance screening was applied to screen actinomycin D high-producting strain. After testing the resistance of Streptomyce rubiginosohelvolus FIM-N31 to streptomycin, the lethal concentration of streptomycin was determined. A lot of streptomycin-resistant mutants were screened after the spores regenerated on the lethal streptomycin concentration (50 μg/mL) media when they were treated with microwave irradiation. It was eventually found that the high-yield strain Str186 from these mutants which was stably inheritable, and the productivity was beyond eight times higher than the original strains in the rotation-flask experiments.

Key words: Streptomyces, actinomycin D, antibiotics resistance gene screening, compound mutation

任林英,张祝兰,王徳森,杨煌建,唐文力,孙菲. 微波诱变结合抗性突变筛选放线菌素D高产菌株[J]. 生物技术进展, 2015, 5(5): 377-381.

REN Lin-ying, ZHANG Zhu-lan*, WANG De-sen, YANG Huang-jian, TANG Wen-li, SUN Fei. Screening of Actinomycin D High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis[J]. Curr. Biotech., 2015, 5(5): 377-381.

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微波诱变结合抗性突变筛选放线菌素D高产菌株

Screening of Actinomycin D High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis

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