关键词: 阿维菌素, 阿维链霉菌, 诱变, 理性筛选

Abstract: Spores and protoplasts of the original strain Streptomyces avermitilis 76-12 were treated with nitrosoguanidine (NTG), acridine orange, ultraviolet radiation (UV) and LiCl respectively. Then a series of high yield avermectin producing and stable mutants were obtained through mutation and rational selection. The avermection productivity of the mutant N-1-2 was 2.47 times of the original strain. Furthermore, several special mutants were obtained. Mutant strain G-32 produced only “a” avermectins; mutant strain Ave8 had higher lever of avermectin B1a content than original strain; and mutant strain UA-G developed bluegreen spores.

Key words: avermectin, streptomyces avermitilis, mutation, rational screening