重组胶原蛋白妇科凝胶对大鼠宫颈炎的治疗作用

doi:10.19586/j.2095-2341.2023.0129

生物技术进展 ›› 2024, Vol. 14 ›› Issue (1): 42-47.DOI: 10.19586/j.2095-2341.2023.0129

• 生物制品研发与技术专题 • 上一篇下一篇

重组胶原蛋白妇科凝胶对大鼠宫颈炎的治疗作用

刘鑫(), 李华, 齐磊, 杨利霞, 于月欣, 徐兰举()

河北纳科生物科技有限公司，石家庄 050000

收稿日期:2023-10-17 接受日期:2023-11-28 出版日期:2024-01-25 发布日期:2024-02-05
通讯作者: 徐兰举
作者简介:刘鑫 E-mail: lxin912@163.com；
基金资助:
河北省引进国外智力项目

Therapeutic Effect of Recombinant Collagen Gynecological Gel on Cervicitis in Rats

Xin LIU(), Hua LI, Lei QI, Lixia YANG, Yuexin YU, Lanju XU()

Hebei NACOL Biotechnology Co. ，Ltd. ，Shijiazhuang 050000，China

Received:2023-10-17 Accepted:2023-11-28 Online:2024-01-25 Published:2024-02-05
Contact: Lanju XU

摘要/Abstract

摘要：

宫颈炎是一种多发的妇科疾病，给越来越多的女性带来困扰。临床上多采用抗生素治疗宫颈炎，该方式的不良反应较大。将重组胶原蛋白开发成局部外用的妇科凝胶剂，用于大鼠宫颈炎的治疗。实验利用25%苯酚胶浆诱导SD大鼠宫颈炎动物模型，再将重组胶原蛋白妇科凝胶以局部给药的方式注入阴道进行治疗，观察宫颈炎模型鼠外阴红肿情况及脓性分泌物的变化。采用苏木素-伊红（hematoxylin-eosin，HE）及马松（masson）染色检查大鼠组织病理变化和胶原沉积情况，通过免疫组化实验检测组织中IL-6、Ki-67蛋白的表达水平，以评价各治疗组对阴道和宫颈组织中炎症反应的调节及促细胞增殖作用。结果发现，与正常组相比，模型组大鼠体重明显减轻（P<0.05），阴道口红肿、有脓性分泌物流出（P<0.05），宫颈组织出现细胞变性、坏死脱落，大量炎性细胞浸润，IL-6蛋白表达水平显著上调（P<0.05），Ki-67表达水平降低（P<0.05）。与模型组相比，治疗后阳性对照组、GG01组、GG02组大鼠体重显著增加（P<0.05），阴道口红肿及分泌物量显著减轻（P<0.05），炎性细胞浸润明显减少，无明显水肿和充血现象，组织中显示胶原沉积及有序排列，且GG02组治疗效果更显著；GG02组和阳性对照组中IL-6和Ki-67表达水平与正常组接近，炎症反应降低，组织受损细胞增殖水平恢复。研究结果表明重组胶原蛋白妇科凝胶对大鼠急性宫颈炎有良好的治疗作用，为其临床应用提供了数据支持。

关键词: 重组胶原蛋白, 妇科凝胶, 宫颈炎, 动物模型

Abstract:

Cervicitis is a kind of frequent gynecological disease， which brings trouble to more and more women. Antibiotics are often used in clinical treatment of cervicitis， which has great side effects. In this study， recombinant collagen was developed into a topical gynecological gel and applied to the treatment of cervicitis in rats. 25% phenol glue was used to induce cervicitis animal model in SD rats， and then recombinant collagen gynecological gel was injected into vagina by local administration for treatment. The changes of vulva swelling and purulent secretion in cervicitis model rats were observed. Hematoxylin-eosin（HE） and masson staining were used to examine the histopathologic changes and collagen deposition in rats. The expression levels of IL-6 protein and Ki-67 protein in the tissues were detected by immunohistochemical experiments. The regulation of inflammatory response and promoting cell proliferation were evaluated in the vaginal and cervical tissues of each group. Results showed that， compared with normal group， the body weight of rats in model group was significantly reduced （P<0.05）. Vaginal opening was red， swollen， and purulent secretions flowed out （P<0.05）. Cervical tissues showed cell degeneration， necrosis and exfoliation， and a large number of inflammatory cells infiltrated. The expression of IL-6 protein was significantly up-regulated （P<0.05）， and the expression of Ki-67 was decreased （P<0.05）. Compared with model group， the body weight of rats in positive control group， GG01 group and GG02 group was significantly increased after treatment（P<0.05）. The swelling and secretions of the vaginal opening were significantly reduced （P<0.05）， the inflammatory cell infiltration was significantly reduced， there was no obvious edema and congestion， collagen deposition and orderly arrangement were shown in the tissues， and the treatment effect of GG02 group was more significant. The expression levels of IL-6 and Ki-67 in GG02 group and positive control group were close to those in normal group， the inflammatory response was reduced， and the proliferation level of damaged tissue cells was restored. The recombinant collagen gynecological gel has a good therapeutic effect on acute cervicitis in rats and provides data support for its clinical application.

Key words: recombinant collagen, gynecological gel, cervicitis, animal model

中图分类号:

Q291

刘鑫, 李华, 齐磊, 杨利霞, 于月欣, 徐兰举. 重组胶原蛋白妇科凝胶对大鼠宫颈炎的治疗作用[J]. 生物技术进展, 2024, 14(1): 42-47.

Xin LIU, Hua LI, Lei QI, Lixia YANG, Yuexin YU, Lanju XU. Therapeutic Effect of Recombinant Collagen Gynecological Gel on Cervicitis in Rats[J]. Current Biotechnology, 2024, 14(1): 42-47.

图/表 5

图1 苯酚胶浆诱导的SD大鼠宫颈炎模型阴道外观

Fig. 1 Vaginal appearance of the rat model of cervicitis induced by phenol mucilage

表1 大鼠体重及白细胞总数指标的比较

Table 1 Comparison of body weight and total WBC in rats

组别	体重变化/g		白细胞总数（/×10⁹·L^-1）
组别	实验前	实验后	白细胞总数（/×10⁹·L^-1）
正常组	192.17±3.38	244.87±7.14	9.50±1.08
模型组	192.63±2.42	202.97±8.00*	20.40±2.22*
阳性对照组	193.48±7.73	237.99±6.45*#	10.30±1.16#
GG00组	191.49±2.96	212.70±8.92*#	14.20±0.79*#
GG01组	191.35±3.41	227.06±6.68#&'	12.30±0.95#&'
GG02组	192.81±4.17	235.29±7.57*#	10.30±1.34#*'

图2 大鼠宫颈组织HE和Masson染色结果A：HE染色结果；B：Masson染色结果

Fig. 2 HE and Masson staining results of rat cervical tissues

图3 大鼠宫颈组织Ki-67和IL-6免疫组化结果A:Ki-67免疫组化结果：B：IL-6免疫组化结果

Fig. 3 Immunohistochemical results of Ki-67 and IL-6 in rat cervical tissue

表2 各组大鼠阴道及宫颈组织Ki-67蛋白及IL-6蛋白指标的比较

Table 2 Comparison of Ki-67 protein and IL-6 protein indexes in vaginal and cervical tissues of rats in each group

蛋白名称	组别	IRS评分
Ki-67	正常组	10.80±1.93
	模型组	1.80±0.42*
	阳性对照组	9.60±2.07#
	GG00组	2.50±1.08*
	GG01组	6.60±0.97#&'
	GG02组	11.20±1.69#*'
IL-6	正常组	0.90±0.57
	模型组	8.80±1.69*
	阳性对照组	1.30±0.67#
	GG00组	8.00±1.63*
	GG01组	5.60±0.84#&'
	GG02组	1.20±0.79#*'

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重组胶原蛋白妇科凝胶对大鼠宫颈炎的治疗作用

Therapeutic Effect of Recombinant Collagen Gynecological Gel on Cervicitis in Rats

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