生物检测试剂保藏技术研究进展

doi:10.19586/j.2095-2341.2023.0110

生物技术进展 ›› 2024, Vol. 14 ›› Issue (1): 94-101.DOI: 10.19586/j.2095-2341.2023.0110

• 生物制品研发与技术专题 • 上一篇下一篇

生物检测试剂保藏技术研究进展

刘莹莹(), 赵可心, 王艺霏, 路凯旋, 胡孔新()

中国检验检疫科学研究院，北京 100176

收稿日期:2023-09-14 接受日期:2023-11-28 出版日期:2024-01-25 发布日期:2024-02-05
通讯作者: 胡孔新
作者简介:刘莹莹 E-mail: liuyybd@126.com；
基金资助:
国家重点研发计划项目(2021YFC2600503);北京市科技计划项目(Z221100005222007)

Research Progress of Biological Detection Reagent Preservation Technology

Yingying LIU(), Kexin ZHAO, Yifei WANG, Kaixuan LU, Kongxin HU()

Chinese Academy of Inspection and Quarantine，Beijing 100176，China

Received:2023-09-14 Accepted:2023-11-28 Online:2024-01-25 Published:2024-02-05
Contact: Kongxin HU

摘要/Abstract

摘要：

生物检测试剂的扩增性能，尤其是酶试剂的生物活性对荧光定量PCR等的检测结果起决定性作用。采用不同的保藏技术对维持检测试剂的生物活性，实现生物检测试剂室温条件下长时间稳定保藏与非冷链运输，保证检测结果的稳定性、准确性、特异性和灵敏度等至关重要。针对低温保藏技术、真空冷冻干燥技术、喷雾干燥技术以及玻璃化冷冻技术4种不同保藏技术的研究进展进行了综述，在应用中总结优缺点，以期对后续相关工作的开展提供一定的科学依据。

关键词: 生物检测试剂, 低温保藏技术, 真空冷冻干燥技术, 喷雾干燥技术, 玻璃化冷冻技术

Abstract:

The amplification performance of biological detection reagents， especially the biological activity of enzyme reagents， plays a decisive role in the detection results of real-time quantitative PCR. It is crucial to adopt different preservation technologies to maintain the biological activity of detection reagents， achieve long-term stable preservation and non-cold chain transportation of biological detection reagents at room temperature， and ensure the stability， accuracy， specificity and sensitivity of detection results. This paper reviewed the research progress of four different preservation technologies， including vacuum freeze-drying technology， spray drying technology， vitrification freezing technology， and summarized the advantages and disadvantages in application， in order to provide a certain scientific basis for the follow-up related work.

Key words: biological detection reagents, cryogenic preservation technology, vacuum freeze-drying technology, spray drying technology, vitrification freezing technology

中图分类号:

Q-33

刘莹莹, 赵可心, 王艺霏, 路凯旋, 胡孔新. 生物检测试剂保藏技术研究进展[J]. 生物技术进展, 2024, 14(1): 94-101.

Yingying LIU, Kexin ZHAO, Yifei WANG, Kaixuan LU, Kongxin HU. Research Progress of Biological Detection Reagent Preservation Technology[J]. Current Biotechnology, 2024, 14(1): 94-101.

图/表 1

参考文献 52

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保藏技术类型	应用场景	优点	缺点
低温保藏技术	生物制品、医疗、食品加工和保藏、日常生活等领域	有效保持核酸检测试剂的活性，延长保藏期限，同时能够确保试剂在运输和使用过程中的稳定性	需要使用专业的冷藏设备，如果设备故障或保存不当，可能导致试剂失效，且冷链物流运输成本高昂
真空冷冻干燥技术	生物制药、疫苗研发与生产、诊断试剂、食品等领域	可实现常温保藏与运输，同时能够保持试剂的稳定性和活性，技术成熟稳定	操作时间长、设备相对较昂贵、运行成本较高，技术操作复杂
喷雾干燥技术	生物医药、食品、化学、农药、催化剂等领域	可实现常温保藏与运输，产品密度低、比表面积高、流动性好、稳定性高、可溶性好且粒径可控	操作时间相对较短、过程较复杂、投资成本高
玻璃化冷冻技术	生物制药、生物酶、细胞与胚胎、组织、食品等领域	可实现常温保藏与运输，储存稳定性好、复苏率高	操作较复杂、投资成本较高

保藏技术类型	应用场景	优点	缺点
低温保藏技术	生物制品、医疗、食品加工和保藏、日常生活等领域	有效保持核酸检测试剂的活性，延长保藏期限，同时能够确保试剂在运输和使用过程中的稳定性	需要使用专业的冷藏设备，如果设备故障或保存不当，可能导致试剂失效，且冷链物流运输成本高昂
真空冷冻干燥技术	生物制药、疫苗研发与生产、诊断试剂、食品等领域	可实现常温保藏与运输，同时能够保持试剂的稳定性和活性，技术成熟稳定	操作时间长、设备相对较昂贵、运行成本较高，技术操作复杂
喷雾干燥技术	生物医药、食品、化学、农药、催化剂等领域	可实现常温保藏与运输，产品密度低、比表面积高、流动性好、稳定性高、可溶性好且粒径可控	操作时间相对较短、过程较复杂、投资成本高
玻璃化冷冻技术	生物制药、生物酶、细胞与胚胎、组织、食品等领域	可实现常温保藏与运输，储存稳定性好、复苏率高	操作较复杂、投资成本较高

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生物检测试剂保藏技术研究进展

Research Progress of Biological Detection Reagent Preservation Technology

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