油菜耐盐碱种质鉴定与品种选育研究进展

doi:10.19586/j.2095-2341.2022.0104

生物技术进展 ›› 2022, Vol. 12 ›› Issue (5): 647-654.DOI: 10.19586/j.2095-2341.2022.0104

油菜耐盐碱种质鉴定与品种选育研究进展

高桂珍¹(), 翟云孤², 张鲁斌¹, 常金梅¹, 罗海华¹, 伍晓明²()

^1.嘉应学院生命科学学院，广东省山区特色农业资源保护与精准利用重点实验室，广东梅州 514015
^2.中国农业科学院油料作物研究所，农业农村部油料作物生物学与遗传育种重点开放实验室，武汉 430062

收稿日期:2022-06-15 接受日期:2022-08-05 出版日期:2022-09-25 发布日期:2022-09-30
通讯作者: 伍晓明
作者简介:高桂珍E-mail：gaoguiz0610@163.com；
基金资助:
中国农业科学院科技创新工程重大科研任务(CAAS-ZDRW202105);广东省自然科学基金项目(2021A1515012523)

Advances on Germplasm Identification and Variety Breeding to Saline-alkali Stress of Rapeseed

Guizhen GAO¹(), Yungu ZHAI², Lubin ZHANG¹, Jinmei CHANG¹, Haihua LUO¹, Xiaoming WU²()

^1.Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas，School of Life Science，Jiaying University，Guangdong Meizhou 514015，China
^2.Key Laboratory of Biology and Genetic Improvement of Oil Crops，Ministry of Agriculture and Rural Affairs，Oil Crops Research Institute of Chinese Academy of Agricultural Sciences，Wuhan 430062，China

Received:2022-06-15 Accepted:2022-08-05 Online:2022-09-25 Published:2022-09-30
Contact: Xiaoming WU

摘要/Abstract

摘要：

盐碱地作为有效的耕地后备资源，其整治和利用尤为重要。油菜是世界上重要的油料作物，也是用于开发利用盐碱地的重要作物之一。从渗透调节物质、抗氧化酶活性、光合作用参数以及盐碱胁迫相关基因报道等方面分析了油菜响应盐碱胁迫的生理生化和分子机制，全面阐述了油菜苗期、营养生长期和成熟期耐盐碱种质的鉴定方法和优异资源筛选，概述了耐盐碱油菜品种选育和推广利用的最新状况，并对未来的研究方向进行了展望，为油菜耐盐碱新品种培育提供理论基础和育种思路。

关键词: 油菜, 耐盐碱性, 生理机制, 分子机制, 鉴定, 品种选育

Abstract:

As an effective reserve land resource of cultivated land， the regulation and utilization of saline alkali land is particularly important. Rapeseed is an important oil crop in the world， which is one of the few crops used to develop and utilize in saline-alkali fields. In this paper， the physiological， biochemical and molecular mechanisms of rapeseed response to saline alkali stress were analyzed from the aspects of osmotic regulators， antioxidant enzyme activities， photosynthetic parameters and reports of genes related to saline alkali stress. The identification methods were comprehensively expounds and excellent germplasm of saline alkali tolerant were screened during the seedling stage， vegetative growth stage and mature stage of rapeseed. The latest status of variety selection and utilization of saline alkali tolerant rapeseed were summarized， and the research direction in the future was prospected. It was expected to provide the theoretical basis and breeding ideas for the cultivation of new saline-alkali tolerant varieties in rapeseed.

Key words: rapeseed, saline-alkali tolerant, physiological mechanism, molecular mechanisms, identification, variety breeding

中图分类号:

S565.4

高桂珍, 翟云孤, 张鲁斌, 常金梅, 罗海华, 伍晓明. 油菜耐盐碱种质鉴定与品种选育研究进展[J]. 生物技术进展, 2022, 12(5): 647-654.

Guizhen GAO, Yungu ZHAI, Lubin ZHANG, Jinmei CHANG, Haihua LUO, Xiaoming WU. Advances on Germplasm Identification and Variety Breeding to Saline-alkali Stress of Rapeseed[J]. Current Biotechnology, 2022, 12(5): 647-654.

参考文献 88

1	杨阳.盐碱地中国潜在的耕地资源[J].中国农村科技,2018(11):8-13.
2	毛庆莲,王胜.国内盐碱地治理趋势探究浅析[J].湖北农业科学,2020,59(S1):302-306.
3	万林生,孙红芹,倪正斌,等.油菜盐油杂3号耐盐性试验及沿海滩涂全程机械化栽培技术[J].浙江农业科学,2017,5(6):959-961.
4	张晓松,朱基钗,杜尚泽.大河奔涌奏响新时代澎湃乐章——习近平总书记考察黄河入海口并主持召开深入推动黄河流域生态保护和高质量发展座谈会纪实[J].绿色中国,2021(21):8-13.
5	刘东洋,徐接亮,张凤华.不同油菜品种对盐碱土壤理化性质与微生物多样性的影响[J].新疆农业科学,2019,56(2):246-257.
6	万林生,孙红芹,倪正斌,等.油菜盐油杂3号耐盐性试验及沿海滩涂全程机械化栽培技术[J].浙江农业科学,2017,5(6):959-961.
7	BANAEI-ASL F, BANDEHAGH A, ULIAEI E D, et al.. Proteomic analysis of canola root inoculated with bacteria under salt stress[J]. J. Proteom., 2015, 124:88-111.
8	杨文元,李腾飞,董博,等.播量对麦后复种油菜生物产量及耕层土壤养分的影响[J].西北农业学报,2017,26(4):583-587.
9	吴海卿,黄茂勋,常景礼.油菜、棉花双移栽改良中度盐渍土效果研究[J].土壤通报,2000,31(1):36-38.
10	GUO H J, HUANG Z J, LI M Q, et al.. Growth, ionic homeostasis, and physiological responses of cotton under different salt and alkali stresses[J/OL]. Sci. Rep., 2020, 10(1): 21844[2022-07-20]. .
11	麻莹,曲冰冰,郭立泉,等.盐碱混合胁迫下抗碱盐生植物碱地肤的生长及其茎叶中溶质积累特点[J].草业学报,2007,16(4):25-33.
12	刘杰,张美丽,张义,等.人工模拟盐、碱环境对向日葵种子萌发及幼苗生长的影响[J].作物学报,2008,34(10):1818-1825.
13	刘建新,刘瑞瑞,贾海燕,等.外源H2S对盐碱胁迫下裸燕麦幼苗叶片渗透胁迫的调节作用[J].生态学杂志,2020,39(12):3989-3997.
14	KHAN M A, UNGAR I A, SHOWALTER A M. Effects of salinity on growth,water relations and ion accumulation of the subtropical perennial halophyte,Atriplex griffithii var.stocksii [J]. Ann. Bot., 2000, 85: 225-232.
15	KALAJI H M, JAJOO A, OUKARROUM A, et al.. Chlorophyllafluorescence as a tool to monitor physiological status of plants under abiotic stress conditions[J/OL]. Acta Physiol. Plant, 2016, 38(4):102[2022-07-20].
16	TAVAKKOLI E, FATEHI F, COVENTRY S, et al.. Additive effects of Na⁺and Cl^-ions on barley growth under salinity stress[J]. J. Exp. Bot., 2011, 62(2): 2189-2203.
17	LI R, SHI F, FUKUDA K. Interactive effects of various salt and alkali stresses on growth,organic solutes,and cation accumulation in a halophyte Spartina alterniflora (Poaceae)[J]. Environ. Exp. Bot., 2010, 68(1): 66-74.
18	CAPULA-RODRÍGUEZ R, VALDEZ-AGUILAR L A, CARTMILL D L, et al.. Supplementary calcium and potassium improve the response of tomato (Solanum lycopersicum L.)to simultaneous alkalinity, salinity and boron stress[J]. Commun. Soil Sci. Plant Anal., 2016, 47(4): 505-511.
19	GIANPIERO M, ROBERTA P, ALESSANDRA T, et al.. Use of MSAP markers to analyze the effects of salt stress on DNA methylation in rapeseed (Brassica napus var. oleifera)[J/OL]. PLoS ONE, 2013, 8(9): e75597[2022-07-20]. .
20	张相锋,杨晓绒,焦子伟.植物耐盐性评价研究进展及评价策略[J].生物学杂志,2018,35(6):39-44.
21	YANG C W, SHI D C, WANG D L. Comparative effects of salt and alkali stresses on growth,osmotic adjustment and ionic balance of an alkali-resistant halophyte Suaeda glauca (Bge.)[J]. Plant Growth Regul., 2008, 56(2):179-190.
22	YANG C W, CHONG J, LI C,et al.. Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana during adaptation to salt and alkali conditions[J]. Plant Soil, 2007, 294(1-2):263-276.
23	MUNNS R. Comparative physiology of salt and water stress[J]. Plant Cell Environ., 2002, 25: 239-250.
24	FARHOUDI R, SHARIFZADEH F, POUSTINI K, et al.. The effects of NaCl priming on salt tolerance in canola (Brassica napus) seedlings grown under saline conditions[J]. Seed Sci. Technol., 2007, 35(3):754-759.
25	CHAKRABORT Y K, BOSE J, SHABALA L, et al.. Difference in root K⁺ retention ability and reduced sensitivity of K⁺-permeable channels to reactive oxygen species confer differential salt tolerance in three Brassica species[J]. J. Exp. Bot., 2016, 67(15): 4611-4625.
26	LIU J, CAI H, LIU Y,et al.. A study on physiological characteristics and comparison of salt resistance of two Medicago sativa at the seedling stage[J]. Acta Pratacul. Sin., 2015,22(3): 250-256.
27	FARHOUDI R. Effect of Salt stress on physiological and morphological parameters of rapeseed cultivars[J]. Adv. Environ. Biol.,2011,5(8): 2501-2508.
28	YILDIZ M, AKÇALI N, TERZI H. Proteomic and biochemical responses of canola (Brassica napus L.) exposed to salinity stress and exogenous lipoic acid[J]. J. Plant Physiol., 2015, 179: 90-99.
29	刘铎,丛日春,党宏忠,等.柳树幼苗渗透调节物质对中、碱性钠盐响应的差异性[J].生态环境学报,2014,23(9):1531-1535.
30	DOLATABADIAN A, SANAVY S M, CHASHMI N. The effects of foliar application of ascorbic acid (vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of canola (Brassica napus L.) under conditions of salt stress[J]. J. Agron. Crop Sci., 2008, 194: 206-213.
31	XUE X N, LIU A H, HUA X J. Proline accumulation and transcriptional regulation of proline biosynthesis and degradation in Brassica napus [J]. BMB Rep., 2009,42(1):28-34.
32	DEINLEIN U, STEPHAN A B, HORIE T, et al.. Plant salt-tolerance mechanisms[J]. Trends Plant Sci., 2014, 19(6): 371-379.
33	王佺珍,刘倩,高娅妮,等.植物对盐碱胁迫的响应机制研究进展[J].生态学报,2017,37(16):5565-5577.
34	SPERDOULI I, MOUSTAKAS M. Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress[J]. J. Plant Physiol., 2012, 169(6): 577-585.
35	贾旭梅,朱燕芳,王海.垂丝海棠应对盐碱复合胁迫的生理响应研究[J].生态学报,2019,39(17):6349-6361.
36	ADAMS P, THOMAS J C, VERNON D M, et al.. Distinct cellular and organismic responses to salt stress[J]. Plant Cell Physiol., 1992, 33(8): 1215-1223.
37	杨洋,王亚娟,阴法庭,等.盐碱胁迫对油菜苗期生理及光合特性的影响[J].北方园艺,2020(15):1-8.
38	DAS K, ROYCHOUDHURY A. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants[J/OL]. Front. Environ. Sci., 2014, 2: 53[2022-06-20]. .
39	AHANGER M A, AZIZ U, ALSAHLIA A, et al.. Influence of exogenous salicylic acid and nitric oxide on growth, photosynthesis, and ascorbate-glutathione cycle in salt stressed Vignaangularis [J]. Biomolecules, 2019, 10(1): 42.
40	FOYERC H, NOCTOR G. Redox signaling in plants[J]. Antioxidants Redox Signal, 2013, 18: 2087-2090.
41	JULKOWSKAM M, TESTERINK C. Tuning plant signaling and growth to survive salt[J]. Trends Plant Sci., 2015, 20(9): 586-594.
42	RANGANI J, PARIDA A K, PANDA A, et al..Coordinated changes in antioxidative enzymes protect the photosynthetic machinery from salinity induced oxidative damage and confer salt tolerance in an extreme Halophyte salvadora persica L.[J]. Front. Plant Sci., 2016,7(537): 50.
43	李兵兵,魏小红,徐严.麻花秦艽种子休眠机制及破除方法[J].生态学报,2013,33(15):4631-4638.
44	YANG J Y, ZHENG W, TIAN Y, et al.. Effects of various mixed salt-alkaline stresses on growth, photosynthesis, and photosynthetic pigment concentrations of Medicago ruthenica seedling[J]. Photosynthetica, 2011, 49(2): 275-284.
45	李学孚,倪智敏,吴月燕,等.盐胁迫对'鄞红'葡萄光合特性及叶片细胞结构的影响[J].生态学报,2015,35(13):4436-4444.
46	严青青,张巨松,代健敏.甜菜碱对盐碱胁迫下海岛棉幼苗光合作用及生物量积累的影响[J].作物学报,2019,45(7):1128-1135.
47	HUANG L, LI Z, PAN S, et al.. Ameliorating effects of exogenous calcium on the photosynthetic physiology of honeysuckle (Lonicera japonica) under salt stress[J]. Funct. Plant Biol., 2019, 46(12): 1103-1113.
48	高立杨,贾旭梅,朱祖雷,等.盐碱复合胁迫下2种长富2号苹果砧穗组合的光合及生理特性[J].干旱地区农业研究,2020,38(2):177-184.
49	孙璐,周宇飞,李丰先,等.盐胁迫对高粱幼苗光合作用和荧光特性的影响[J].中国农业科学,2012,45(16):3265-3272.
50	李辛,赵文智.荒漠区植物雾冰藜光合特性对混合盐碱胁迫的响应[J].生态学报,2018,38(4):1183-1193.
51	KAYUM M A, JUNGH J, PARK J I, et al.. Identification and expression analysis of WRKY family genes under biotic and abiotic stresses in Brassica rapa [J]. Mol. Genet. Genom., 2015, 290: 79-95.
52	KIM J A, JUNG H E, HONG J K, et al.. Reduction of GIGANTEA expression in transgenic Brassica rapa enhances salt tolerance[J]. Plant Cell Rep., 2016, 35(9): 1943-1954.
53	LIS T, ZHANG L, WANG Y, et al.. Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli[J]. Sci. Rep., 2017, 7: 41397.
54	杜坤,高亚楠,孔月琴,等.转入OsLTP对甘蓝型油菜耐盐水平的影响[J].中国农业科学,2013,46(13):2625-2632.
55	皮明雪.BnTR1在甘蓝型油菜抗旱和耐盐中的功能鉴定[D].扬州:扬州大学,2017.
56	贺亚军,吴道明,游婧璨,等.油菜耐盐相关性状的全基因组关联分析及其候选基因预测[J].中国农业科学,2017,50(7):1189-1201.
57	ZHONG H, GUO Q Q, CHEN L, et al.. Two Brassica napus genes encoding NAC transcription factors are involved in response to highsalinity stress[J]. Plant Cell Rep., 2012,31:1991-2003.
58	GHARELO S R, BANDEHAGH A, TOURCHI M, et al.. Canola 2-dimensional proteome profile under osmotic stress and inoculation with Pseudomonas fluorescens FY32[J]. Plant Cell Biotechnol. Mol. Biol., 2016, 17: 257-266.
59	JIA H, SHAO M, HE Y, et al.. Proteome dynamics and physiological responses to short-term salt stress in Brassica napus leaves[J/OL]. PLoS ONE, 2015, 10(12):e0144808[2022-08-05]. .
60	于崧,张婷婷,于立河,等.盐碱胁迫对小麦种子萌发特性的影响[J].黑龙江八一农垦大学学报,2019,31(2):20-27.
61	许耀照,曾秀存,方彦,等.盐碱胁迫对油菜种子萌发和根尖细胞有丝分裂的影响[J].干旱地区农业研究,2014,32(4):14-19.
62	PARIDA A K, DAS A B. Salt tolerance and salinity effects on plants: a review[J]. Ecotoxicol. Environ. Saf., 2005, 60: 324-349.
63	丁富功,卢奕霏,康珍,等.混合盐碱胁迫对油菜种子萌发和幼苗生长的影响[J].长江大学学报(自然科学版),2020,17(3):73-80, 90.
64	闫改各,周建.外源水杨酸对盐碱胁迫下海滨锦葵生长、Na^＋富集与转运的影响[J].河南科技学院学报(自然科学版),2019,47(4):10-15.
65	龙卫华,浦惠明,张洁夫,等.甘蓝型油菜发芽期的耐盐性筛选[J].中国油料作物学报,2013,35(3):271-275.
66	李萍,燕佳琦,张鹤,等.146份甘蓝型油菜种质芽期耐盐性筛选及评价[J].西北农业学报, 2021,30(6):848-859.
67	王治红,刘自刚,孙万仓,等.NaCl和Na₂SO₄胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析[J].干旱地区农业研究,2016,34(6):243.
68	王洁,孟秋峰,任锡亮.等 .十字花科作物耐盐种质研究现状及展望[J].现代农业科技,2019,(12):60-63.
69	吴杨,高慧纯,张必弦,等.24-表油菜素内酯对盐碱胁迫下大豆生育、生理及细胞超微结构的影响[J].中国农业科学,2017,50(5):811-821.
70	崔世友,张蛟蛟.沿海滩涂野生叶用芥菜的耐盐性及利用潜力[J].江苏农业科学,2014,42(12):397-398.
71	WANI A S, AHMAD A, HAYAT S, et al.. Salt-induced modulation in growth, photosynthesis and antioxidant system in two varieties of Brassica juncea [J]. Saudi J. Biol. Sci., 2013, 20: 183-193.
72	PITANN B, SCHUBERT S, MÜHLING K H. Decline in leaf growth under salt stress is due to an inhibition of H⁺-pumping activity and increase in apoplastic pH of maize leaves[J]. J. Plant Nutr. Soil Sci., 2009, 172(4): 535-543.
73	HAYAT S, MAHESHWARI P, WANI A S, et al.. Comparative effect of 28 homobrassinolide and salicylic acid in the amelioration of NaCl stress in Brassica juncea L.[J]. Plant Physiol. Biochem., 2012, 53:61-68.
74	KAUR H, SIRHINDI G, BHARDWAJ R, et al.. 28-homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt- and temperature-induced oxidative stress in Brassica juncea [J/OL]. Sci. Rep., 2018, 8(1): 8735[2022-06-20]. .
75	MUNNS R. Comparative physiology of salt and water stress[J]. Plant Cell Environ., 2002, 25(2): 239-250.
76	VALIOLLAH R. Effect of salinity stress on yield, component characters and nutrient compositions in rapeseed (Brassica napus L.) genotypes[J]. Agric. Trop. Subtrop., 2013, 46(2): 58-63.
77	CHAKRABORTY K, SAIRAM R K, BHADURI D. Effects of different levels of soil salinity on yield attributes accumulation of nitrogen, and micronutrients in Brassica spp. [J]. J. Plant Nutr., 2016, 39(7):1026-1037.
78	龙卫华,胡茂龙,陈松,等.盐地种植对甘蓝型油菜产量和品质性状的影响[J].江苏农业科学,2015,43(3):85-87.
79	BYBORDI A. Effects of salinity on yield and component characters in canola (Brassica napus L.) cultivars[J]. Not. Sci. Biol., 2010, 2(1): 81-83.
80	ZADEH H M, BNAEINI M B. Effects of salinity stress on themorphology and yield of two cultivars of canola (Brassica napus L.)[J]. J. Agronomy, 2007, 6(3):409-414.
81	朱孔志,吴明昊,申玉香,等.不同油菜品种在盐碱地的耐盐性鉴定及筛选[J].浙江农业科学,2018,59(8):1354-1356.
82	ZAMANI S, BYBORDI A, KHORSHIDI M B,et al.. Effects of NaCl salinity levels on lipids and proteins of canola(Brassica napus L.) cultivars[J]. Adv. Environ. Biol.,2010,4(3):397-403.
83	汪波,文静,张凤华,等.耐盐碱油菜品种选育及修复利用盐碱地研究进展[J].科技导报,2021,39(23):59-64.
84	胡茂龙,浦惠明,陈新军,等.双低杂交油菜宁杂15号耐盐性鉴定[J].江苏农业科学,2011,39(2):144-146.
85	万林生,孙红芹,倪正斌,等.油菜盐油杂3号耐盐性试验及沿海滩涂全程机械化栽培技术[J].浙江农业科学,2017,58(6):959-961.
86	万何平,戴希刚,陈敬东,等.甘蓝型油菜对盐胁迫的响应及耐盐相关性状QTL研究进展[J].中国油料作物学报,2020,42(4):536-544.
87	夏龙珠,朱鹏飞,高群山,等.苏中盐碱地区机直播油菜品种比较[J].中国农技推广,2018,34(11):32-34.
88	崔军军,王美娥,陈明,等.沿海盐碱地区机械直播油菜品种筛选研究[J].上海农业科技,2017(4):57-58.

[1]	咸志慧, 龙卫华, 谭筱玉, 胡茂龙, 浦惠明. 高油酸油菜遗传育种研究进展[J]. 生物技术进展, 2022, 12(5): 641-646.
[2]	杨美翠, 赵诗慧, 高源, 石浩然, 李云, 龚万灼, 杨进, 王继胜, 邹琼, 陶兰蓉, 康泽明, 唐蓉, 郭世星, 付绍红. 油菜双单倍体诱导育种技术研究进展[J]. 生物技术进展, 2022, 12(5): 655-663.
[3]	陶鸽如, 秦树存. 氢生物医学效应在疏解自由基氧化应激的分子机制[J]. 生物技术进展, 2022, 12(4): 490-496.
[4]	张文静, 佟晔, 杨锡文, 曹彦金, 魏计东. 高产中性蛋白酶菌株的筛选、优化及中试放大[J]. 生物技术进展, 2022, 12(1): 112-119.
[5]	肖荣, 魏云晓, 王远, 孟志刚, 梁成真, 陈全家, 张锐. 茎尖法转基因棉花植株真实性鉴定方法探究[J]. 生物技术进展, 2022, 12(1): 83-89.
[6]	咸莉梅, 胡怡, 李磊, 孙政玺, 何心尧, 李韬. 浅议小麦赤霉病抗性类型与鉴定方法的对应性[J]. 生物技术进展, 2021, 11(5): 554-559.
[7]	翟文玲, 刘彩云, 刘颖, 付必胜, 蔡瑾, 郭炜, 张巧凤, 吴纪中. 小麦赤霉病新抗源的发掘与抗性位点的检测分析[J]. 生物技术进展, 2021, 11(5): 581-589.
[8]	高佳奇,陈硕,王迪,龙艳,李亮,张晓. 六种作物内标准基因开发与检测研究进展[J]. 生物技术进展, 2020, 10(6): 613-622.
[9]	徐石勇,赵新,张富丽,刘征辉,史清洪,宋君,王永,兰青阔. 基于焦磷酸测序分析技术的金银花掺伪鉴别方法研究[J]. 生物技术进展, 2020, 10(6): 637-645.
[10]	陈硕,高佳奇,王迪,龙艳,李亮,张晓. DNA四面体纳米结构及其在生物技术领域的应用进展[J]. 生物技术进展, 2020, 10(6): 661-667.
[11]	钟彩霞,田佳雪,王晓琪,温彤. 一株番茄表面着生醋酸菌的分离鉴定及产酸条件优化[J]. 生物技术进展, 2020, 10(5): 564-569.
[12]	张红兵,刘荟,史秀英,李会宣,范道春. 产油微藻的选育及其培养条件优化[J]. 生物技术进展, 2020, 10(3): 311-319.
[13]	栗旭阳,黄丽玲,郭倩楠,高如雨,张维,陈明,陆伟,周正富. 除草剂2,4-D降解菌株的分离、筛选与鉴定[J]. 生物技术进展, 2019, 9(4): 384-395.
[14]	陈泽历,杨林毅,陈潞,孙雁,魏朝霞,李永忠,赵明富,文国松. 侵染滇黄精的菜豆普通花叶病毒的检测与鉴定[J]. 生物技术进展, 2019, 9(2): 122-128.
[15]	邓展瑞,贠建民,郭娟,牛耀星,李彦虎. 陇西腊肉加工过程中优势乳酸菌的分离及其发酵性能研究[J]. 生物技术进展, 2019, 9(2): 200-209.

油菜耐盐碱种质鉴定与品种选育研究进展

Advances on Germplasm Identification and Variety Breeding to Saline-alkali Stress of Rapeseed

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 88

相关文章 15

编辑推荐

Metrics

本文评价