关键词: 微藻, 筛选, 鉴定, 油脂含量, 响应面优化

Abstract:

It is necessary to screen microalgae strains that can adapt to the climate of Shijiazhuang because the feasibility of using microalgae in wastewater treatment as a supplement for wastewater treatment can couple between environmental pollution control and the alleviation of fossil energy shortage. In this paper, 10 microalgae strains were selected after purification from rivers in Shijiazhuang city by using BG11 medium. The data of all microalgae strains culture were shown that the DK-3 was the best one than others with the high biomass(1.86 g·L-1) and the high total fat content \[(10.23±2.63) mg·L-1·d-1\] under the autotrophic condition by variance analysis with SPSS 19.0 and LSD multiple comparison. The DK-3 was identified as Scenedesmus meyen and the phylogenetic tree was constructed by 18S rDNA sequencing analysis. In order to explore the optimal heterotrophic conditions of algae DK-3, the experiment scheme was designed by couple between Box-Behnken design (BBD) and response surface method (RSM). The result showed that the best culture condition of DK-3 was the BG11 with glucose 20 g·L-1, K2HPO4·3H2O 90 mg·L-1, and MgSO4·7H2O 200 mg·L-1, respectively. Compared with the biomass (3.37 g·L-1) of unoptimized group, the biomass (5.47 g·L-1) and oil yield (1.28 g·L-1) of DK-3 under the optimized conditions above was increased by 62.31% after 240 h training. Therefore, the DK-3 is a good algae with a high research potential.

Key words: microalgae, screening, appraisal, oil content, response surface optimization