关键词: 芒果炭疽病, 胶孢炭疽菌, 柯赫氏法则, 生防细菌筛选

Abstract: With the increasing demand for mango from Yunnan, the planting area of mango has been expanding, and new varieties are continuously introduced, thus causing the occurrence of anthracnose disease of mango in Yunnan Province to become more and more serious. In order to carry out targeted biological control of mango anthracnose, the pathogen of postharvest mango anthracnose was isolated by tissue block separation method. Morphological observation was used as preliminary identification, and the pathogen was verified according to Koch’s postulates. Subsequently, rDNA-ITS sequence and phylogenetic tree analysis were used to identify the taxonomic status of pathogenic bacteria. Finally, 5 kinds of biocontrol bacteria were used to conduct antagonistic tests on pathogenic bacteria. Through isolation and identification of the pathogen causing mango postharvest anthracnose, Colletotrichum gloeosporioides was determined to be the pathogen causing mango postharvest anthracnose in Yunnan Province. The sequence length of internal transcribed spacer (ITS) in this strain was 536 bp and the login number was MH744668. The five biocontrol bacteria had certain bacteriostatic to mango anthracnose pathogenic bacteria, and had good biocontrol development potential. Among them, the antibiotic L-44 had the best bacteriostatic effect, with the inhibition rate of 53.7%. The research results provided a new insight into biological control of mango postharvest diseases in Yunnan Province.

Key words: mango anthracnose disease, Colletotrichum gloeosporioide, Koch’s postulates, biocontrol bacterium screening