脂肪酶研发态势的全球专利分析

doi:10.19586/j.2095-2341.2020.0161

生物技术进展 ›› 2021, Vol. 11 ›› Issue (6): 749-757.DOI: 10.19586/j.2095-2341.2020.0161

脂肪酶研发态势的全球专利分析

张亚格¹^,²(), 庞雨¹, 张维¹, 周正富¹()

^1.中国农业科学院生物技术研究所，农业农村部农业基因组学重点实验室，北京 100081
^2.海南省农业科学院三亚研究所，海南三亚 572000

收稿日期:2020-12-17 接受日期:2021-05-24 出版日期:2021-11-25 发布日期:2021-11-26
通讯作者: 周正富
作者简介:张亚格 E-mail：zhangyage1992@163.com；
基金资助:
国家自然科学基金项目(31930004);国家科技重大专项(2019ZX08010-002);中国农业科学院科技创新工程重大科研任务(CAAS- ZDRW202009)

Global Patent Analysis of Lipase Development Trends

Yage ZHANG¹^,²(), Yu PANG¹, Wei ZHANG¹, Zhengfu ZHOU¹()

^1.Key Laboratory of Agricultural Genomics，Ministry Agriculture and Rural Affairs，Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China
^2.Sanya Institute，Hainan Academy of Agricultural Sciences，Hainan Sanya 572000，China

Received:2020-12-17 Accepted:2021-05-24 Online:2021-11-25 Published:2021-11-26
Contact: Zhengfu ZHOU

摘要/Abstract

摘要：

脂肪酶作为一种能够催化各种酯键断裂和形成的绿色环保生物催化剂，已被广泛应用于食品、饲料、洗涤剂、制药、精细化学以及生物修复等领域。基于智慧芽数据库（Pat Snap）对2020年10月前全球范围内各个国家及地区的脂肪酶相关专利进行统计，分析脂肪酶的技术分布、发展状况和未来趋势，并对比分析我国的脂肪酶研发现状，以期为脂肪酶技术及产业发展方向提供参考依据。

关键词: 脂肪酶, 专利, 研发态势, 洗涤剂

Abstract:

Lipase is a kind of green and environment?friendly biocatalyst which can catalyze the break and formation of various ester bonds. Lipases have been widely used in many fields such as food， feed， detergent， pharmaceutical， fine chemistry and bioremediation. Based on Pat Snap， statistic on lipase?related patents in various countries and regions around the world before October 2020， we analyzed the lipase technology distribution， development status and future trends， and comparative analyzed the lipase research and development in China to provide a reference for the development of lipase technology and industry.

Key words: lipase, patent, research and development situation, detergent

中图分类号:

张亚格, 庞雨, 张维, 周正富. 脂肪酶研发态势的全球专利分析[J]. 生物技术进展, 2021, 11(6): 749-757.

Yage ZHANG, Yu PANG, Wei ZHANG, Zhengfu ZHOU. Global Patent Analysis of Lipase Development Trends[J]. Current Biotechnology, 2021, 11(6): 749-757.

图/表 10

图1 国内外脂肪酶专利数量申请趋势

Fig.1 Trend of the lipase patent applications number at home and abroad

图2 脂肪酶专利技术来源国家/地区/组织分布

Fig.2 Country/region/organization distribution of lipase patent technology sources

图3 主要技术来源国家的年度申请趋势

Fig.3 Annual application trends of major technology source countries

图4 主要专利受理国家/地区/组织分布

Fig.4 Distribution of major patent acceptance countries/regions/organization

图5 全球脂肪酶专利重要IPC分布

Fig.5 Important IPC distribution of global lipase patents

表1 IPC分类号的定义

Table 1 Definition of IPC classification number

IPC分类号	定义
C12N9	酶，如连接酶；酶原；其组合物；制备、活化、抑制、分离或纯化酶的方法
A61K31	含有机有效成分的医药配制品
C12N15	突变或遗传工程；遗传工程涉及的DNA或RNA，载体（如质粒）或其分离、制备或纯化；所使用的宿主
C11D3	包括在C11D1/00组内洗涤组合物的其他配料成分
A61K38	含肽的医药配制品
C12P7	含氧有机化合物的制备
A61P3	治疗代谢疾病的药物（治疗血液或细胞外液的A61P7/00）
C12N1	微生物本身，如原生动物；其组合物；繁殖、维持或保存微生物或其组合物的方法；制备或分离含有一种微生物组合物的方法；其培养基
C12Q1	包含酶、核酸或微生物的测定或检验方法；其组合物；组合物的制备方法
C07K14	具有多于20个氨基酸的肽；促胃液素；生长激素释放抑制因子；促黑激素；其衍生物

图6 脂肪酶专利重要技术领域的国家分布

Fig.6 Country distribution of important technical fields of lipase patents

图7 全球脂肪酶专利重要申请机构

Fig.7 Important global application institutions of lipase patents

图8 脂肪酶专利重要技术领域的主要研发机构分布

Fig.8 Distribution of main research institutions in important technical fields of lipase patents

图9 国内脂肪酶专利的情况A：中国脂肪酶专利申请前10位机构的专利数量；B：国内脂肪酶专利法律状态

Fig.9 Status of lipase patents in China

参考文献 30

1	BORNSCHEUER U T. Microbial carboxyl esterases: classification, properties and application in biocatalysis[J]. FEMS Microbiol. Rev., 2002, 26(1):7381.
2	JAEGER K E, EGGERT T. Lipases for biotechnology[J]. Curr. Opin. Biotechnol., 2002, 13(4):390-397.
3	徐碧林,朱庆.微生物脂肪酶稳定性研究进展[J].微生物学通报,2020,47(6):1958-1972.
4	HAKI G D, RAKSHIT S K. Developments in industrially important thermostable enzymes: a review[J]. Bioresour. Technol., 2003, 89(1):17-34.
5	JOSEPH B, RAMTEKE P W, THOMAS G, et al.. Standard review cold-active microbial lipases: a versatile tool for industrial applications[J]. Biotechnol. Mol. Biol. Rev., 2007, 2(2):39-48.
6	施慧琳,王玥,李祯祺,等.从专利角度分析全球酶制剂研发态势[J].生物产业技术,2019(03):5-12.
7	陈雄金.脂肪酶的研究及其应用[J].科技资讯,2008(19):58.
8	KANMANI P, ARAVIND J, KUMARESAN K. An insight into microbial lipases and their environmental facet[J]. Int. J. Environ. Sci. Technol., 2015, 12(3):1147-1162.
9	SAISUBRAMANIAN N. New tools for exploring "old friends—microbial lipases"[J]. Appl. Biochem. Biotechnol., 2012, 168(5): 1163-1196.
10	李香春.脂肪酶的研究进展[J].肉类工业,2003(4):45-48.
11	SHOZO Y. A process for producing lipase: UK, GB238507A[P] 1926-07-14.
12	UNILEVER L, DETERGENT C. Detevgent compositions: UK, GB1372034A [P]. 1974-10-30.
13	HASAN F, SHAH A A, HAMEED A. Industrial applications of microbial lipases[J]. Enzyme Microb. Technol., 2006,39:235-251.
14	KAPOOR M, GUPTA M N. Lipase promiscuity and its biochemical applications[J]. Proc. Biochem., 2012, 47: 555569.
15	LI X L, ZHANG W H, WANG Y D, et al.. A high-detergent-performance, cold-adapted lipase from Pseudomonas stutzeri PS59 suitable for detergent formulation[J]. J. Mol. Catal. B Enzym., 2014,102: 16-24.
16	NERURKAR M, JOSHI M, PARITI S, et al.. Application of lipase from marine bacteria bacillus sonorensis as an additive in detergent formulation[J]. J. Surfact. Deterg., 2013, 16(3):435-443.
17	龚俊勇,吴伟坚,左建军.脂肪酶和乳化剂对AA肉鸡生长性能、表观消化率和腹脂率的影响[J].饲料工业,2019,40(10):40-46.
18	GERITS L R, PAREYT B, DELCOUR J A. A lipase based approach forstudying the role of wheat lipids in bread making[J]. Food Chem., 2014, 156: 190-196.
19	KONKIT M, KIM W. Activities of amylase, proteinase, and lipase enzymes from Lactococcus chungangensis and its application in dairy products[J]. J. Dairy Sci., 2016, 99(7): 1-9.
20	BORNSCHEUER U T. Enzymes in lipid modification: From classical biocatalysis with commercial enzymes to advanced protein engineering tools[J]. Ocl-OL. Corps Gras Li., 2013, 20(1): 45-49.
21	JAEGER K E, EGGERT T. Lipases for biotechnology[J]. Curr. Opin. Biotechnol., 2002, 13(4): 390-397.
22	JOSEPH B, RAMTEKE P W, THOMAS G, et al.. Standard review cold-active microbial lipases: a versatile tool for industrial applications[J]. Biotechnol. Mol. Biol. Rev., 2007, 2(2): 39-48.
23	HASAN F, SHAH A A, HAMEED A. Industrial applications of microbial lipases[J]. Enzyme Microb. Technol., 2006, 39: 235-251.
24	AHMED E H, RAGHAVENDRA T, MADAMWAR D. An alkaline lipase from organic solvent tolerant Acinetobacter sp. EH28: application for ethyl caprylate synthesis[J]. Bioresour. Technol., 2010, 101(10): 3628-3634.
25	BHUSHAN I, KUMAR A, MODI G, et al.. Chiral resolution of differently substituted racemic acetyl-1-phenyl ethanol using lipase from Bacillus subtilis[J]. J. Chem. Technol. Biotechnol., 2011, 86: 315-318.
26	郁惠蕾,张志钧,李春秀,等.大数据时代工业酶的发掘、改造和利用[J].生物产业技术,2016,2:48-55.
27	HAKI G D, RAKSHIT S K. Developments in industrially important thermostable enzymes: a review[J]. Bioresour. Technol., 2003, 89(1):17-34.
28	肖文海,王颖,元英进.化学品绿色制造核心技术——合成生物学[J].化工学报,2016(1):119-128.
29	叶纯,王玉娟.脂肪酶的生物学改造研究进展[J].生物学杂志, 2020,37(01):81-84.
30	金城.微生物酶工程:绿色生物制造的基石[J].微生物学报,2020,47(7):2001-2002.

[1]	党星, 郅斌伟, 曹克浩, 刘婷婷, 陈飚, 丁远杰. 转基因玉米生物育种技术的专利分析及产业发展建议[J]. 生物技术进展, 2022, 12(4): 614-622.
[2]	杜梦潭,刘兴健,胡小元,张志芳,李轶女. 腺相关病毒的生产方式及其在基因治疗中的应用[J]. 生物技术进展, 2019, 9(4): 326-331.
[3]	王宇洲,马锐,向杰,陈敬师,李世贵,龚明波,顾金刚. Trichoderma lentiforme ACCC30425脂肪酶基因的原核表达及其酶学性质的初步研究[J]. 生物技术进展, 2018, 8(6): 530-536.
[4]	陈静,冷鹃,杨喜爱,廖丽萍,肖爱平,刘亮亮. 磁性纳米粒子固定化酶技术研究进展[J]. 生物技术进展, 2017, 7(4): 284-289.
[5]	王凯,刘洪国,姜昆,闫培生. 产脂肪酶深海细菌的筛选鉴定及酶学性质研究[J]. 生物技术进展, 2015, 5(3): 241-245.
[6]	李有朝,蔡玉品,郝佳. 埃博拉病毒疫苗研究进展及相关专利浅析[J]. 生物技术进展, 2015, 5(2): 142-147.
[7]	杨威,杜伟,刘德华. 游离脂肪酶催化蓖麻油制备蓖麻油酸[J]. 生物技术进展, 2014, 4(5): 373-378.
[8]	孙菲，郑榕，杨煌建，张祝兰. 胰脂肪酶抑制剂产生菌的筛选[J]. 生物技术进展, 2014, 4(1): 40-43.

脂肪酶研发态势的全球专利分析

Global Patent Analysis of Lipase Development Trends

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 30

相关文章 8

编辑推荐

Metrics

本文评价