关键词: 丙酮丁醇梭菌, 丁醇, 原生质体, 融合

Abstract: The study was airmed to improve the butanol tolerance of Clostridium acetobutylicum and increase the conversion rate of total sugar in culture medium to produce butanol by the protoplasmic fusion method. The effect of  concentration of lysozyme and its action time, the type of regenerative medium, the time of thermal death at 55 ℃, different PEG molecular weights and the action time, Ca2+, Mg2+ additions with different amounts on Clostridium acetobutanol protoplasm preparation, fusion, regeneration was studied. We obtained a set of comparative system of Clostridium acetobutanol protoplasm fusion conditions. And we detected the solvent production capacity of the fusion bacteria by gas chromatography and calculated the total sugar conversion rate. Results showed that, the total sugar conversion rate of the final obtained strain 215I was 34.7% higher than the original strain, and the butanol production capacity was 32.2% higher than the original strain, and one fusion strain was found to produce new substances. The protoplasmic fusion method is promising in the breeding of Clostridium acetobutylicum, and the strains obtained by fusion provide a basis for further experiments.

Key words: Clostridium acetobutylicum, butanol, protoplasts, fusion