关键词: 玉米皮, 丁醇发酵, 拜氏梭菌

Abstract: Corn fiber, a byproduct of corn starch processing, is a potential unexpensive bioresource for butanol production. Butanol production from corn fiber using Clostridium beijerinckii NCIMB 8052 was investigated in this study. Corn fiber was firstly pretreated by 0.5% sulfuric acid with solid\|to\|liquid ratio of 1：8 for 20 min under the optimal temperature 140℃，followed by enzymatic hydrolysis at glucoamylase loading of 200 IU/g（dry material）and xylanase loading of 1.0 IU/g （dry material）. The starch and hemicellulose were mostly hydrolyzed into fermentable sugars including glucose，xylose and arabinose，resulting in a total fermentable sugars concentration of 50.46 g/L. Then the hydrolysate of corn fiber was detoxified by 1.0% active carbon in order to remove the inhibitors of butanol fermentation. Batch fermentation using the detoxified hydrolysate of corn fiber showed that 9.72 g/L of butanol and 14.09 g/L of total solvents were produced at the end of the fermentation period，and the sugar-to-solvents ratio was 35.1%. These results above suggested that it is a feasible strategy for butanol production by corn fiber fermentation using Clostridium beijerinckii NCIMB 8052.

Key words: corn fiber, butanol fermentation, Clostridium beijerinckii NCIMB 8052