关键词: 碳纳米管, 固定化, 纤维素酶, 固定化酶活力

Abstract: Cellulase has been widely used in environmental protection, medicine, food and other fields. However, the widespread use of cellulase was limited by its relatively high production costs and low biological activity. In this study, in order to provide a method for immobilizing cellulase that allowed the enzyme to be reused in multiple cycles, multi-walled carbon nanotubes was used as carriers to immobilize cellulase for the first time. The conditions for immobilization of enzyme with multi-walled carbon nanotubes were studied. An orthogonal experimental design was applied to optimize critical conditions for the immobilization process. The structures of MWCNTs, cellulase and immobilized cellulase were characterized by Fourier transform infrared (FT-IR) spectroscopy. The results showed that the optimum process conditions for immobilized enzyme were as follows: enzyme concentration 5 mg·mL-1, temperature 40 ℃, pH 5.0, immobilization time 3 h, and the immobilization of cellulase was confirmed successfully onto the multi-walled carbon nanotubes by Fourier transform infrared spectroscopy.

Key words: carbon nanotubes, immobilization, cellulase, the activity of immobilized enzyme