关键词: 计量学, 流式细胞术, 激光诱导荧光检测, 数字聚合酶链式反应

Abstract: Developing accurate and sensitive nucleotide quantification method to improve the detection capability for traceable nucleotide samples, is of great importance under the background of fighting against present COVID-19 epidemic. Flow cytometry counting for single nucleotide molecule is a type of numeration-based quantification method, which counts the copy number of target fluorescence labelled-nucleotide molecules without PCR amplification, tracing directly to international system of unit. As a potential primary metrological method, it is an important supplement for the present nucleotide quantification method, and could be widely applied for many fields, such as clinical diagnostics, genetic-modified organism labelling and detection of food & drug residues. Here the principal, hardware constitution of the apparatus, data analysis model were reviewed, and the significance to biometric standard system was also prospected.

Key words: metrology, flow cytometry, laser-induced fluorescence detection, digital PCR