关键词: 金黄色葡萄球菌, PCR鉴定体系, 水产品

Abstract: Staphylococcus aureus is an important pathogen which can cause food poisoning. To simplify the complicated detecting method of Staphylococcus aureus and understand the prevalence in aquatic product of Zhoushan area, this paper compared 3 PCR identification systems of Staphylococcus aureus. According to the specificity and sensitivity of PCR identification systems, one optimal PCR identification system of Staphylococcus aureus was selected. Based on the selected method and national standard approach, we investigated the prevalence of Staphylococcus aureus in 120 samples collected from the aquatic products processing factory of Zhoushan city. The result showed that the positive rate of Staphylococcus aureus contamination among the 120 samples was 8.3%, and there was no significant difference in positive rate between PCR method and national standard approach. The detected positive samples were focused on the raw material and the environment, but not in the semi-finished and finished aquatic products. The results indicated that the finished aquatic products was relatively safe, but a certain degree of security hidden danger existed in the raw material and the environment of the aquatic products processing factory.

Key words: Staphylococcus aureus, PCR identification system, aquatic products