关键词: 棉花, 线粒体, 环式RT-PCR, 细胞质雄性不育

Abstract: Plant mitochondria is a type of organelles containing genetic material，which provides necessary energy for cells. In agricultural production, genovariation of mitochondrial gene may lead to cytoplasmic male sterility, providing a convenient female parent for hybird breeding. Despite successful utilization of CMS and restoration of fertility (Rf) in breeding programs, the underlying mechanisms of these processes remain elusive because the mitochondrial genome structure is complex, and occurrence of different types of CMS is often caused by specific mutations consequently limiting gene cloning. This study summarized the techniques to improve cRT-PCR technology and present detailed characteristics mitochondrial gene involved in CMS. After RNA ligase cyclizes the 5′ and 3′ ends of mitochondrial mRNA, random primers were used for reverse transcription to ensure the same integrity as specific primers. Based on the mitochondrial gene transcript, cRT-PCR was performed on all mitochondrial coding genes, which provided insight of structure of mitochondrial coding genes. Therefore, we used this system to explore cotton CMS genes, thus quickly figuring out abnormal factors and providing a new method for mitochondrial functional genomic analysis.

Key words: cotton, mitochondrial, cRT-PCR, CMS