关键词: 木霉, 固态发酵, 响应面法, 玉米芯, 酒精

Abstract: A strain W2 was separated from the rotted cotton of Hubei province. According to morphology and 18S rDNA sequences analysis, strain W2 belongs to Trichoderma sp. Corn cob was used as substate to optimize solid-state fermentation (SSF) conditions for CMCase production. Four parameters including fermention time, temperature, initial moisture content and pH were optimized and the CMCase activity was acted as response value. The interaction of the respective factors and their influences on CMCase were studied by Box-Benhnken central composite design and response surface methodology, the quadratic equation and response figures were established. The optimal fermentation conditions were obtained based on single-factor tests. Under culture temperature of 25℃, initial moisture content of 63% and pH 6.0, CMCase achieved its high value after 65h fermentation. At last, synchronous fermentation was adopted to produce alcohol, and the alcohol content reached 0.38 mg/mL determined by improved Conway method.

Key words: Trichoderma, solid-state fermention, response surface methodology, corn cob, alcohol