关键词: PRRSV, CD163, 克隆, 原核表达

Abstract: In order to prokaryotically express the CD163 protein, one receptor of porcine reproductive and respiratory syndrome virus (PRRSV), we predicted the structure of CD163 using ScanProsite software, and fragmented it. CD163 fragments from primary alveolar macrophages (PAM) were amplified by RT-PCR using specific primers based on the known sequence from pig PRRSV(GenBank accession no. EU016226). Three fragments of CD163 were amplified and cloned into the prokaryotic expression vector pET-28a (+), the recombination proteins of CD163 fragments were expressed in E. coli cells. Western blot test verified that the expressed recombinant proteins had good antigen activities.This work provided the experimental basis for further study of the CD163 receptors role in the process of viral infection, and the interaction with other receptors.

Key words: PRRSV, CD163, clone, prokaryotic expression