关键词: 重组抗狂犬病毒单克隆抗体, N-糖链切除, 毛细管电泳, 中和活性

Abstract: The recombinant anti-rabies monoclonal antibody were fully-human-derived antibody and were used for post- explosure prophylaxis of rabies virus. The N-glycan of two anti-rabies monoclonal antibodies (Mab1 and Mab2) were hydrolyzed by the PNGase in this study, the capillary electrophoresis and gel staining by periodic acid- schiff method were used to determine the residual N-glycan after hydrolysis. The rapid fluorescent focus inhibition test was used to compare the neutralization bio-activity of the intact Mab1 and the Mab2 without N-glycan. The result showed that the neutralization bioactivity of  Mab1 and Mab2 did not change after the N- glycan was removed from the antibodies. That suggests the N-glycan was not the indispensable composition for in-vitro neutralization bioactivity of Mab1 and Mab2.

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