关键词: 葛根素, 非酒精性脂肪肝, 小鼠, 保护作用, 肝细胞脂肪变性

Abstract:

Nowadays， non-alcoholic fatty liver disease （NAFLD） has become an important research object of liver-related diseases， and the prevalence of NAFLD in China has been increasing year by year. However， the treatment options for NAFLD are still relatively limited， and traditional Chinese medicine has played an advantageous role in its treatment. In this experiment， forty healthy male mice were randomly divided into five groups of eight mice each： normal group， model group， low-dose group， medium-dose group， and high-dose group. The model of NAFLD was prepared by high-fat feeding for 35 days according to the improved modeling method， except for the normal group. From day 36 onwards， the low-dose， medium-dose and high-dose groups （60， 80， 100 mg·kg^-1） were gavaged with geraniol dissolved in saline， and the rest of the groups were gavaged with saline according to the formula of gavage volume. After 16 days of administration， triglyceride （TG）， total cholesterol （TC）， high-density lipoprotein （HDL）， low-density lipoprotein （LDL） indexes were tested， and the livers of the mice were removed to prepare pathological sections. The experimental results showed that the middle and high doses of puerarin had a more significant lowering effect on the lipid-related indexes （TC， TG， LDL） of the NAFLD model mice， and the effect of the high dose of puerarin in lowering TG was more obvious （P<0.01）. The experimental results suggested that puerarin may have a certain protective effect on NAFLD in mice.

Key words: puerarin, nonalcoholic fatty liver disease, mice, protective effect, hepatocyte steatosis