关键词: 创伤性颅脑损伤, 吸氢, 急性炎症反应, 神经保护作用

Abstract: In order to investigate the effect of hydrogen inhalation on inflammatory response in acute phase of traumatic brain injury (TBI) in rats, 6-week-old male SD rats were randomly divided into sham operation group, TBI group and hydrogen inhalation treatment group. The level of serum cytokines was detected by suspension chip technology at 2, 6 and 24 h after TBI, and the neuroprotective effect of hydrogen inhalation was evaluated by modified neurological severity score (mNss) at 24 h after TBI. Meanwhile, the brain tissue was taken for Nissl staining analysis and serum biochemical indexes were detected. The results of mNss score showed that the neurological function of TBI rats was significantly improved within 24 h after hydrogen inhalation. The protective effect of hydrogen inhalation on neurons was further confirmed by Nissl staining. The results of serum cytokines detection showed that hydrogen inhalation could exert inhibitory effect on  acute inflammation induced by TBI, evidenced by remarkable reduction in seven pro-inflammatory cytokines in serum at 2 h after TBI. In addition, hydrogen inhalation markedly decreased serum levels of cardiac and hepatic biomarkers, suggesting that hydrogen inhalation could attenuate cardiac and hepatic function impairment in the acute phase of TBI. Studies suggested that hydrogen inhalation may play a neuroprotective role by inhibiting the inflammatory response in the acute phase of TBI.

Key words: traumatic brain injury, hydrogen inhalation, acute inflammation, neuroprotective effect