关键词: 幽门螺旋杆菌, 胃炎模型, 小鼠

Abstract: The establishment of stable and reliable mouse gastritic model using Helicobacter pylori (Hp) standard strains has important significance in the research of Hp vaccine development, Hp pathogenesis and screening of anti-Hp drugs. In this study, the BALB/c mouse gastritic models were constructed by international standard strain Hp ATCC 43504. Then the BALB/c mouse gastritic models were analyzed by Hp urease activity detection, Hp quantitative culture, PCR detection and histopathological staining methods. The mice gastric tissues from BALB/c mouse gastritic models could make Hp urease reagent appear rose red by analysis of Hp diagnostic kit. But mice gastric tissues from control group could not enable Hp change color and still appear yellow. Hp could grow up at BHI blood plate by Hp quantitative culture using gastric homogenate from BALB/c mouse gastritic models, but Hp could not emerge at BHI blood plate by Hp quantitative culture using gastric homogenate from control group. 150 bp DNA products could be amplified from gastric tissue of BALB/c mice in the model group by PCR, while the control group was no amplification products. HE staining method was used to observe the pathological changes and inflammation in mice. Compared with BALB/c mice from the control group, there was a large number of white blood cell infiltration in the gastric mucosa from the model group. BALB/c mouse gastritic models were constructed using Hp ATCC 43504, which could be used to evaluate the effect of the drug on the prevention and treatment of Hp infection.

Key words: Helicobacter pylori, gastritic model, mice