双分子荧光互补技术在动物病毒中的研究进展

doi:10.19586/j.2095-2341.2022.0048

生物技术进展 ›› 2022, Vol. 12 ›› Issue (6): 825-836.DOI: 10.19586/j.2095-2341.2022.0048

双分子荧光互补技术在动物病毒中的研究进展

周水娟(), 郭忠建()

江苏大学生命科学学院，江苏镇江 212000

收稿日期:2022-04-02 接受日期:2022-06-30 出版日期:2022-11-25 发布日期:2022-11-30
通讯作者: 郭忠建
作者简介:周水娟 E-mail: 2807948441@qq.com；
基金资助:
国家自然科学基金项目(31770174)

Progress of Bimolecular Fluorescence Complementation Technology in Animal Viruses Research

Shuijuan ZHOU(), Zhongjian GUO()

Shool of Life Science，Jiangsu University，Jiangsu Zhenjiang 212000，China

Received:2022-04-02 Accepted:2022-06-30 Online:2022-11-25 Published:2022-11-30
Contact: Zhongjian GUO

摘要/Abstract

摘要：

病毒侵染宿主的过程存在着一系列相互作用，了解病毒与宿主之间的蛋白质相互作用对于深入研究病毒具有重要意义。在众多研究蛋白质相互作用的方法中，双分子荧光互补技术（bimolecular fluorescence complementation，BiFC）因其能在活细胞中可视化相互作用而被广泛应用。介绍了双分子荧光技术的原理、发展和优势，总结了双分子荧光技术在动物病毒以及抗病毒药物研究中的应用，并进一步阐述了新型双分子荧光系统的原理，以期为研究动物病毒致病机制和抗病毒药物研发提供新的思路。

关键词: 双分子荧光互补技术, 蛋白质相互作用, 动物病毒, 抗病毒药物

Abstract:

There are a series of protein-protein interactions in the process of virus infection. Therefore， understanding the interactions between the viruses and the hosts are of great significance for the in-depth studies of virology. Currently， there are many techniques used in protein interactions research， among which the bimolecular fluorescence complementation （BiFC）is widely used due to its ability to visualize interactions in living cells. This paper introduced the principle， development and advantages of BiFC technology. The application of BiFC technology in animal viruses and antiviral drugs were also summarized， and the principle of some new bimolecular fluorescence systems were further elaborated， so as to provide new ideas for the studies of virus pathogenic mechanism and the development of antiviral drugs.

Key words: BiFC, protein-protein interaction, animal viruses, antiviral drugs

中图分类号:

Q939.47

周水娟, 郭忠建. 双分子荧光互补技术在动物病毒中的研究进展[J]. 生物技术进展, 2022, 12(6): 825-836.

Shuijuan ZHOU, Zhongjian GUO. Progress of Bimolecular Fluorescence Complementation Technology in Animal Viruses Research[J]. Current Biotechnology, 2022, 12(6): 825-836.

图/表 6

图1 BiFC技术的原理图

Fig. 1 Principle of BiFC technology

图2 BiFC技术中荧光蛋白和光敏色素发展注：EGFP—增强型绿色荧光蛋白；EBFP—增强型蓝色荧光蛋白；EYFP—增强型黄色荧光蛋白；ECFP—增强型青色荧光蛋白；mRFP1-Q66T—单体红色荧光蛋白变体（Q66T）；Venus—黄色荧光蛋白变体（F46L/F64L/M153T/V163A/S175G）；Citrine—黄色荧光蛋白变体（Q69M）；Cerulean—青色荧光蛋白变体（S72A/Y145A/H148D）；frGFP—折叠报告器GFP；mKG—单体珊瑚荧光报告蛋白；mCherry—樱桃色荧光蛋白；mKate—远红荧光蛋白报告基因；DsRed monomer—红色荧光蛋白单体；TagRFP—橘红色荧光蛋白；mLumin—mKate-S158A变体；Dronpa—单体GFP样荧光蛋白；sfGFP—一种设计精良的折叠版本GFP突变体（S30R/Y39N/N105T/Y145F/I171V/A206V）；GFP-S65T—绿色荧光蛋白突变体（S65T）；iRFP—近红外荧光蛋白；IRF1.4—近红外荧光蛋白1.4；mNeptune—mKate变体（M41G/S61C/S158C/Y194F/）；mScarlet-I—单体红色荧光蛋白变体；Kusabira-Orange(KO)—橙色荧光蛋白；IFP2.0—近红外荧光蛋白2.0；miRFP670namo—最小的近红外荧光蛋白。

Fig. 2 The development of fluorescent proteins and photochromes in BiFC

图3 Nef二聚体与Itk相互作用激活模型[37]注：MHC—组织相容性复合物；TCR—T细胞抗原受体；CD4—表面抗原分化簇4受体；LCK—蛋白酪氨酸激酶；PLC γ—磷脂酶C γ链；DAG—二酰基甘油；IP3—三磷酸肌醇；NF-κB—核转录因子；NFAT—活化T细胞核因子。

Fig. 3 Models of Nef-dimer mediated Itk activation[37]

表1 BiFC在致病性病毒蛋白互作研究中的应用

Table 1 Application of BiFC in the study of proteins interaction of pathogenic viruses

病毒种类	相互作用的蛋白	荧光标签（荧光蛋白和光敏色素）	相互作用定位	参考文献
人类免疫缺陷病毒	IN与importin α	GFP	细胞核	[28]
	IN与LEDGF/p75	iRFP	染色体	[29]
	IN多聚体	Venus	细胞质	[32]
	Nef二聚体	YFP	细胞膜和高尔基体网络	[36]
	Nef与Itk、Btk	Venus	细胞膜	[37]
	Nef与AP-2、SERINC5	Venus	细胞膜	[39]
	Nef与PACS	YFP	细胞质	[40]
	Nef与SNX18	Venus	细胞质	[41]
	Nef与Tim-3	Venus	细胞质	[42]
	Env与Env、SERINC5	Venus	细胞膜和细胞质	[43]
	Vpu与Tim-3	Venus	未知	[44]
	Gag与Gag、AGO2	Venus	未知	[45]
疱疹病毒	gD与gB、gH/gL	Venus	细胞质和细胞核	[46]
	gB与gH/gL	Venus	细胞质和细胞核	[46-47]
	ICP27寡聚体	Venus	细胞核	[48-49]
	ICP27与TAP/NXF1	Venus	细胞核	[50]
	HSRG1与LT	YFP	细胞核	[51]
	LMP1与LMP1、TRAF2、TRAF3	YFP	细胞膜及核周	[53]
	LMP1与Tmem134	YFP	细胞质	[54]
	LMP1与肌动蛋白细胞骨架相关蛋白	YFP	脂筏	[55]
	pUL21与Roadblock-1	Lumin	细胞质	[56]
	pUL14与VP16	Venus	细胞核	[57]
流感病毒	PA与PB1	Venus	细胞核	[60-61]
	PB1与PB2	Venus	细胞核	[60-61]
	PA与PB2	Venus	细胞核	[60-61]
	RIG I与PB2	Venus	细胞核	[62]
	RIG I与PA、PB1	Venus	细胞质	[62]
其他病毒	NS4B与STING	mKG	细胞质	[63]
	E2与Brd4	Venus	细胞核	[64]
	VP2与Apoptin	YFP	细胞核	[66]
	RIG-I与TRIM25、MAVS	Venus	细胞质	[65]
	PRRSV跨膜非结构蛋白互作网络	Venus	未知	[67]
	poMx1与NS5B	Venus	细胞质	[68]
	BmCdc37与BmHsp90	Venus	细胞质	[69]
	Claudin-2与VP7	dsRed	未知	[70]
	SARS-CoV-2结构蛋白和辅助蛋白	Venus	未知	[71]
	SARS-CoV-2核衣壳蛋白与细胞应激颗粒蛋白	miRFP670nano	细胞质	[24]

图4 BAC-BiFC用于PPI成像的工作原理[45]

Fig. 4 Working principle of BAC-BiFC for PPI imaging[45]

图5 tBiFC用于PPI成像的工作原理[22]

Fig. 5 Working principle of tBiFC for PPI imaging[22]

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双分子荧光互补技术在动物病毒中的研究进展

Progress of Bimolecular Fluorescence Complementation Technology in Animal Viruses Research

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