关键词: 茧蜂病毒, 亲环素A, 病毒感染, Hi5细胞

Abstract: The Microplitis bicoloratus bracovirus (MbBV) is one type of polydnavirus, which is mainly found in parasitic wasps (Hymenoptera: Braconidae) and plays a vital role in parasitism. Cyclophilin A (CypA) is a peptidyl-prolyl cis-trans isomerase involved in several different cellular events including the immune response. During the process of insect cells infected by MbBV, whether there was any relationship between MbBV and the level of CypA was mainly investigated. The results showed that the MbBV gene can be detected in Hi5 insect cells after 24 h of infection with MbBV, during which the transcriptional level and protein expression level of cypa were both upregulated. When CypA was silenced or suppressed in MbBV-treated Hi5 cells, vank86, a gene encoding one member of MbBV, was remarkably down-regulated via analysis of qRT-PCR. And overexpression of CypA in Hi5 cells leaded to an upregulation of the vank86. These results indicated that the level of CypA in insect cells might be correlated to the MbBV infection.

Key words: MbBV, cyclophilin A, viral infection, Hi5 cells