生物技术进展 ›› 2014, Vol. 4 ›› Issue (5): 331-339.DOI: 10.3969/j.issn.2095-2341.2014.05.05

• 进展评述 • 上一篇    下一篇

运动发酵单胞菌在生物炼制中的研究进展

何明雄1,2,吴波1,谭芙蓉1,王景丽1,税宗霞1,秦晗1,代立春1,胡启春1,2   

  1. 1.农业部沼气科学研究所, 生物质能技术研究中心, 成都 610041;
    2.农业部农村可再生能源开发与利用重点实验室, 成都 610041
  • 收稿日期:2014-07-28 出版日期:2014-09-25 发布日期:2014-08-15
  • 作者简介:何明雄,副研究员,博士,主要从事微生物代谢工程与生物质能源研究。E-mail:hemingxiong@caas.cn
  • 基金资助:

    国家自然科学基金项目(31000028);新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题基金项目(BRZD1403);山东大学微生物技术国家重点实验室开放基金项目(2013);农业部农业微生物资源收集与保藏重点实验室开放课题基金项目(2013);四川省科技支撑计划项目(2014NZ000045)资助。

Research Progress of Zymomonas mobilis on Biorefinery System

HE Ming-xiong, Wu Bo, Tan Fu-rong, Wang Jing-li,  Shui Zong-xia,  Qin Han,  Dai Li-chun,  Hu Qi-chun,   

  1. 1.Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu 610041, China;
    2.Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Chengdu 610041, China
  • Received:2014-07-28 Online:2014-09-25 Published:2014-08-15

摘要: 以木质纤维素生物质为原料的生物炼制技术已成为全球研发的热点和难点。欧盟国家和美国的中长期生物质能源发展路线图中均将木质纤维素生物炼制技术作为重要目标,但是目前整体水平尚处于中试阶段。我国的纤维素类生物质原料非常丰富,将其转化成燃料乙醇及生物基础化学品等具有较大的潜力,但当前要想实现商业化生产,还面临着很多瓶颈问题亟待解决。缺乏能够同时高效利用纤维素类水解物的发酵菌株,已成为纤维素生物质高效与高值转化的关键制约因素。运动发酵单胞菌是目前唯一一种通过ED途径兼性厌氧发酵葡萄糖的微生物,其独特的代谢途径使其成为构建产乙醇工程菌的优选宿主之一;同时由于该菌具有较高的糖利用效率等优点,也是其他生物基化学品生产的重要候选平台微生物,如山梨醇、葡萄糖酸、丁二酸和异丁醇等。本文从该菌的研究历程、分子生物学基础、菌种改良及该菌在生物能源及生物基化学品等生物炼制体系中的应用研究角度进行了综述,并提出该菌可作为纤维素生物质生物炼制系统的新的重要平台微生物。

关键词: 纤维素生物质, 生物炼制, 燃料乙醇, 生物基化学品, 运动发酵单胞菌

Abstract: Biorefinery technologies using lignocellulosic biomass as feedstock have become a hot topic and huge task in globle research and development,which are also an important goal of long\|term biomass energy roadmap in both EU countries and the United States. However,the current overall level is still in pilot stage. China has abundant biomass resources,which has great potential in the production of biomass energy and bio\|based chemicals,but also faces a lot of bottleneck problems to be solved in order to achieve commercial production. The lack of efficient fermentation strains simultaneously ferment cellulosic hydrolyzate,has become a key constraint in cellulosic biorefinery. Currently,Zymomonas mobilis has became a preferred host in cellulosic ethanol fermentation for its unique ED pathway. Z. mobilis also showed some advantages of higher specific rate of sugar uptake,etc.,which made it an ideal platform for commercial\|scale production of desirable bio\|products,such as sorbitol,gluconic acid,succinic acid and isobutanol. This paper reviewed the research history,molecular biology,strain improvement and its application in biorefinery system, and also put forwards that Z. mobilis could be considered as a noval important microbe platform of cellulosic biomass biorefinery system.

Key words: lignocellulosic biomass, biorefinery, bioethanol, bio-based chemical, Zymomonas mobilis