生物技术进展 ›› 2014, Vol. 4 ›› Issue (5): 340-345.DOI: 10.3969/j.issn.2095-2341.2014.05.06

• 进展评述 • 上一篇    下一篇

嗜热β-甘露聚糖酶的研究进展

王彩虹,牛灿芳,罗会颖*,杨培龙,姚斌   

  1. 农业部饲料生物技术重点实验室, 中国农业科学院饲料研究所, 北京 100081
  • 收稿日期:2014-08-27 出版日期:2014-09-25 发布日期:2014-09-11
  • 通讯作者: 罗会颖,副研究员,博士,主要从事极端环境微生物、极端酶基因挖掘与高效表达研究。E-mail:luohuiying@caas.cn
  • 作者简介:王彩虹,硕士研究生,主要从事微生物代谢与酶工程研究。E-mail:hong19900427@163.com。
  • 基金资助:

    国家自然科学基金项目(31172235)资助。

Progress on Thermophilic β-Mannanase

WANG Cai-hong, NIU Can-fang, LUO Hui-ying, YANG Pei-Long, YAO Bin   

  1. Key Laboratory of Feed Biotechnology,Ministry of Agriculture; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2014-08-27 Online:2014-09-25 Published:2014-09-11

摘要: β-甘露聚糖酶在生物能源、饲料、食品和纺织等工业中均有着重要的应用前景。其属于半纤维素酶类,广泛存在于动植物和微生物中,微生物来源尤为广泛。随着极端微生物和极端酶的广泛研究,嗜热甘露聚糖酶因其在高温环境中具有较高酶活性和稳定性而倍受关注,并取得了较大的研究进展。本文综述了β-甘露聚糖酶的来源、分类和水解催化方式,以及嗜热甘露聚糖酶的优势和其在基因资源挖掘、重组表达以及分子改良方面的研究进展,展望了嗜热β-甘露聚糖酶未来可能的研究方向和发展前景。

关键词: β-甘露聚糖酶, 嗜热酶, 基因资源, 分子改良

Abstract: β-Mannanase has important application prospect in bioenergy,feed, food,textile and other industries. β-Mannanase which belongs to hemicellulose enzymes exists in a wide variety of plants,animals and especially in microorganisms. With the extensive research of extremophiles and extreme enzymes,thermophilic mannanases receive much concern  because of their high enzyme activity and stability at high temperature. This paper reviewed the source and classification of β-mannanases and the hydrolysis pattern of thermophilic β-mannanase,as well the gene cloning,expression and molecular improvement. The research orientation and development prospects were also elucidated in this paper.

Key words: β-mannanases, thermophilic enzyme, genetic resources, molecular improvement