关键词: 角蛋白降解菌, 蛋白水解活性, 最适条件

Abstract: A feather keratindegrading strain (Sporosarcina GIMN1.015) was isolated from soil mixed with feather by enrichment culture. The influences of temperature, initial pH, supplementary carbon sources and feather content on the proteolytic activity were investigated with natural feather as the substrate. The results showed that the proteolytic activity in the feather medium was the highest at 30℃ with initial pH 11.0. Compared with the medium with feathers only, addition of glucose increased proteolytic activity. With the substrate content of 1.5%, the proteolytic activity was highest. The results laid the foundation for further application of feather as resource using keratindegrading microorganisms.

Key words: keratin-degrading strain, proteolytic activity, optimal conditions.