生物技术进展 ›› 2012, Vol. 2 ›› Issue (1): 57-63.DOI: 10.3969/j.issn.2095-2341.2012.01.11
• 技术与方法 • 上一篇 下一篇
张亚旭
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基金资助:
国家863计划项目(2006AA020903)资助。
ZHANG Ya-xu
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摘要： DNA重组技术,即DNA克隆技术的研究和运用是现代生物学发展的一个重要分支,是分子生物学发展的突出领域。本文介绍了DNA重组的类型及相关的生物学概念;综述了目前已报道的传统的酶切-连接经典克隆方法、位点特异性重组克隆方法、以及同源重组克隆方法,重点阐述了各自的原理、步骤、特点及实际应用等方面;最后归纳总结了各种方法的优缺点和应用范围,并对该技术的科研成果进行了回顾和对未来的研究进行了展望。
关键词: DNA重组, 基因克隆, 经典克隆, 位点特异性重组克隆, 同源重组克隆
Abstract: The research and application of DNA recombination technology, i.e. DNA cloning technology is an important branch of the development of modern biology and outstanding area of molecular biology. The paper started from the types and the related biological concepts of DNA recombination, summarized the traditional digestion-ligation classical cloning method, site-specific recombination cloning method and homologous recombination cloning method had been reported at present. The aspects of respective principles, steps, characteristics and practical applications were focused on. Finally, an induction of respective merits and drawbacks and application scopes of these methods and the review for achievements and the prospect for future were described.
Key words: DNA recombination, gene cloning, classical cloning, site-specific recombination cloning, homologous recombination cloning
张亚旭. DNA重组技术的研究综述[J]. 生物技术进展, 2012, 2(1): 57-63.
ZHANG Ya-xu. Research Summary on DNA Recombination Technology[J]. journal1, 2012, 2(1): 57-63.
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