连翘有效成分提取及提取物对奶牛乳房炎致病菌的抑制效果

doi:10.3969/j.issn.2095-2341.2014.04.11

生物技术进展 ›› 2014, Vol. 4 ›› Issue (4): 292-295.DOI: 10.3969/j.issn.2095-2341.2014.04.11

连翘有效成分提取及提取物对奶牛乳房炎致病菌的抑制效果

周艳,张涛*,郑勤琴,杨承霖,樊皓月

天津农学院动物科学与动物医学学院, 天津 300384

收稿日期:2014-06-16 出版日期:2014-07-25 发布日期:2014-07-11
通讯作者: 张涛，讲师，主要从事滨海盐碱地植物资源利用研究。E-mail：zhangtaofood@163.com
作者简介:周艳，本科，研究方向为动物药学。E-mail:845662609@qq.com。
基金资助:
天津农学院大学生科技创新项目资助。

Extracting Active Components of Forsythia suspensa and the Extract‘s Inhibitory Effect on Cow Mastitis Pathogens

ZHOU Yan, ZHANG Tao, ZHENG Qin-qin, YANG Cheng-lin, FAN Hao-yue

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2014-06-16 Online:2014-07-25 Published:2014-07-11

摘要/Abstract

摘要： 中药连翘具有抗菌、消炎、解热等作用，本研究目的在于摸索连翘有效成分提取的试验方法，获得适于抑制奶牛乳房炎致病菌的有效成分。以连翘叶片为材料，利用回流法提取工艺提取，采用薄层色谱（TLC）测定法定性分析，高效液相色谱法（HPLC）测定有效成分纯度，抑菌圈检测法检测抑菌效果。结果表明，连翘提取物对奶牛乳房炎致病菌具有抑制效果。

关键词: 连翘, 成分提取, 奶牛乳房炎, 致病菌, 抑制作用

Abstract: Forsythia suspense has the properties of anti-bacteria， anti-inflammatory， antipyretic and other features. The purpose of this study is to explore the test method of effective component exraction from Chinese medicine Forsythia and explore the component for pathogen inhibiton in cow mastitis. The Forsythia leaves were extracted using reflux extraction technology, the extract was analyzed by TLC, and the purity of the extracted active component was detected by HPLC. Antimicrobial effects of Forsythia suspensa extract was detected using inhibition zone assay. The results showed that extract of Forsythia suspensa had inhibitory effect on dairy cow mastitis pathogens.

Key words: Forsythia suspense, component extraction, cow mastitis, pathogens, inhibition

周艳,张涛,郑勤琴,杨承霖,樊皓月. 连翘有效成分提取及提取物对奶牛乳房炎致病菌的抑制效果[J]. 生物技术进展, 2014, 4(4): 292-295.

ZHOU Yan, ZHANG Tao*, ZHENG Qin-qin, YANG Cheng-lin, FAN Hao-yue. Extracting Active Components of Forsythia suspensa and the Extract‘s Inhibitory Effect on Cow Mastitis Pathogens[J]. Curr. Biotech., 2014, 4(4): 292-295.

[1]	赵文卓, 李成勋, 胡作建, 余红秀. 功能核酸用于致病菌检测的研究进展[J]. 生物技术进展, 2023, 13(1): 30-38.
[2]	安琦, 曹亚彬, 牛彦波, 原韬. 含连翘的青贮饲料对肉鹅饲喂效果研究[J]. 生物技术进展, 2022, 12(2): 265-269.
[3]	卢海强,焦新雅,,吴思源,王娅丽,刘璐,程书梅,张晓,苏晓峰. 数字PCR在食源性致病菌检测中的应用进展[J]. 生物技术进展, 2021, 11(3): 260-268.
[4]	白小莲,爱军. 生物传感器在食源性致病菌大肠杆菌O157∶H7检测中的应用进展[J]. 生物技术进展, 2021, 11(3): 269-278.

连翘有效成分提取及提取物对奶牛乳房炎致病菌的抑制效果

Extracting Active Components of Forsythia suspensa and the Extract‘s Inhibitory Effect on Cow Mastitis Pathogens

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价