生物技术进展 ›› 2013, Vol. 3 ›› Issue (6): 439-442.DOI: 10.3969/j.issn.2095-2341.2013.06.12

• 分离与检测 • 上一篇    下一篇

HPLC-MS/MS法测定树莓中树莓酮含量的研究

李斌1,王小杰2,杨磊1,孟宪军1*   

  1. 1.沈阳农业大学食品学院， 沈阳 110866； 2.阜新桃李园集团公司， 辽宁 阜新 123000
  • 收稿日期:2013-10-08 出版日期:2013-11-25 发布日期:2013-10-30
  • 通讯作者: 孟宪军，教授，博士生导师，研究方向为小浆果 深加工关键技术。E-mail: mengxjsy@126.com
  • 作者简介:李斌，副教授，博士，研究方向为小浆果深加工关键技术。E-mail: libinsyau@163.com。
  • 基金资助:
    农业部公益性行业科研项目 “树莓等初加工及综合利用技术研究示范 ” 项目(201303073-04)资助。

Determination of Raspberry Ketone in Raspberry by HPLC-MS/MS

LI Bin, WANG Xiao-jie, YANG Lei, MENG Xian-jun   

  1. 1.Shenyang Agricultural University, Shenyang 110866, China; 2.Fuxin Tao Li Yuan Company, Liaoning Fuxin 123000, China
  • Received:2013-10-08 Online:2013-11-25 Published:2013-10-30

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摘要： 建立了树莓中树莓酮含量检测的高效液相色谱-串联质谱（HPLC-MS/MS）分 析方法。本研究采用甲醇作为提取溶剂热回流提取树莓酮，通过化学方法除去粗提 物中杂质并分离出其中的醛酮等羰基化合物，色谱分离后进行HPLC-MS/MS多反应监 测模式下的定性及定量分析。结果显示，不同基质中树莓酮的定量检测限为1.0 μg/kg，回收率为754%～82.3%，最大相对标准偏差为8.40%。本方法分析速度快 ，灵敏度高，重现性好，可用于树莓中树莓酮的检测。

关键词: HPLC-MS, 树莓酮 , 树莓

Abstract: A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed for analysis of raspberry ketone in raspberry. Raspberry ketone was extracted by hot refluxing method with methanol as extraction solvent. The impurities in crude extract were removed by chemical method, and the aldehyde, ketone and other carbonyl compounds were isolated from crude extract. Qualitative and quantitative analysis was carried out for the analyte under the MRM mode after the chromatographic separation. The quantitative limit of detection of RK in different matrix was 1.0 μg/kg. The average recovery was 75.4%～82.3%, and the relative standard deviation was 5.97%～8.40%. This method is accurate，rapid，sensitive，and adapt to the inspection of the raspberry ketone in raspberry.

Key words: HPLC-MS/MS, raspberry ketone, raspberry

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