关键词: 灵芝多糖, 超滤, 径向流色谱, 脱蛋白, 截留率

Abstract: The removal of protein and the retention rate of polysaccharide in Ganoderma lucidum polysaccharides hydrolyzate were studied using ultrafiltration and coupling method. Using the ultrafiltration membrane with a cut-off molecular mass of 100 kDa, the effects of several parameters such as the flow rate, ultrafiltration time and concentration multiple on the ultrafiltration were evalutated. The optical conditions of removing protein by ultrafiltrating were determined. It is founded that the flow rate, time and concentration multiple were 100 mL/min, 60 min,15~17 times, respectively, at the room temperature and the pressure less than 30 psi. The rate of protein removal reached 82.21% and the retention rate of polysaccharide was 63.32%. Coupled the ultrafiltration with radial flow chromatography, the retention rate of polysaccharide and the removal rate of protein were as high as 99.24% and 89.52%. As compared with the single chromatography and the Sevag method, the efficiency increased. These findings thus clearly suggested purification of polysaccharides from Ganoderma lucidum by coupling ultrafiltration with radial flow chromatography was feasible with high retention rate.

Key words: Ganoderma lucidum polysaccharides, ultrafiltration, radial flow chromatography, deproteinization, retention rate