免疫磁分离技术在循环肿瘤细胞检测中的研究进展

doi:10.19586/j.2095-2341.2025.0030

生物技术进展 ›› 2025, Vol. 15 ›› Issue (4): 606-614.DOI: 10.19586/j.2095-2341.2025.0030

• 进展评述 • 上一篇

免疫磁分离技术在循环肿瘤细胞检测中的研究进展

赵璐璐¹(), 洪甜¹, 郝一然², 陈尔凝¹, 李静雯¹, 杜美红¹

^1.北京市科学技术研究院分析测试研究所（北京市理化分析测试中心），北京 100094
^2.北京城市学院生物医药学部，北京 101309

收稿日期:2025-03-04 接受日期:2025-05-06 出版日期:2025-07-25 发布日期:2025-09-08
作者简介:赵璐璐 E-mail: zhll2014@yeah.net
基金资助:
北京市科学技术研究院财政资助项目(24CB003-14)

Research Progress of Immunomagnetic Separation Technology in Detection of Circulating Tumor Cell

Lulu ZHAO¹(), Tian HONG¹, Yiran HAO², Erning CHEN¹, Jingwen LI¹, Meihong DU¹

^1.Insititute of Analysis and Testing，Beijing Academy of Science and Technology （Beijing Center for Physical & Chemical Analysis），Beijing 100094，China
^2.School of Biomedicine，Beijing City University，Beijing 101309，China

Received:2025-03-04 Accepted:2025-05-06 Online:2025-07-25 Published:2025-09-08

摘要/Abstract

摘要：

肿瘤侵袭和转移是癌症患者死亡的主要原因，循环肿瘤细胞(circulating tumor cell，CTC)作为肿瘤转移的“种子”细胞，携带完整的细胞生物学信息，在肿瘤的早期诊断、预后评估及个体化治疗监测等方面具有重要意义。然而，由于血液中CTC细胞数量极低，开发高效的富集分离方法是实现CTC精准分析的关键。免疫磁分离技术具有高特异性、高效富集等优势，能够高效、特异性地分离和富集目标物，为生物医学研究和临床诊断提供了强大的工具。随着材料科学、生物技术、电子工程技术等多学科的交叉融合发展，免疫磁分离技术在前沿分析领域中的应用价值得到了显著提升，该技术在CTC检测领域有望迎来重大突破。综述了CTC免疫磁性材料的设计原理、CTC免疫磁分离捕获平台及免疫磁分离技术与微流控联用研究进展，探讨了免疫磁分离技术在CTC检测中的挑战及优化策略，以期为推动CTC检测技术更深入的应用提供参考。

关键词: 循环肿瘤细胞, 免疫磁分离, 免疫磁性复合材料, 微流控芯片

Abstract:

Tumor invasion and metastasis are the primary causes of death in cancer patients. Circulating tumor cell （CTC）， as the seed cells of tumor metastasis， carries complete cellular biological information and hold significant importance in early diagnosis， prognosis assessment， and personalized treatment monitoring of tumors. However， due to the extremely low number of CTC in the blood， developing efficient enrichment and separation methods are crucial for the precise analysis of CTC. Immunomagnetic separation technology， with its advantages of high specificity and efficient enrichment， can effectively and specifically isolate and enrich target substances， providing a powerful tool for biomedical research and clinical diagnosis. As the cross-disciplinary integration and development of materials science， biotechnology， and electronic engineering technology， the application value of immunomagnetic separation technology in cutting-edge analytical areas has been enhanced， expecting to achieve significant breakthroughs in the field of CTC detection. This article reviewed the design of CTC immunomagnetic material， the CTC immunomagnetic separation and capture platform， and the combination of immunomagnetic separation technology with microfluidics， discussed the challenges and optimization strategies of immunomagnetic separation technology in CTC detection， in order to provide a reference for promoting the deeper application of CTC detection technology.

Key words: circulating tumor cell, immunomagnetic separation technology, immunomagnetic composite material, microfluidics

中图分类号:

Q291

赵璐璐, 洪甜, 郝一然, 陈尔凝, 李静雯, 杜美红. 免疫磁分离技术在循环肿瘤细胞检测中的研究进展[J]. 生物技术进展, 2025, 15(4): 606-614.

Lulu ZHAO, Tian HONG, Yiran HAO, Erning CHEN, Jingwen LI, Meihong DU. Research Progress of Immunomagnetic Separation Technology in Detection of Circulating Tumor Cell[J]. Current Biotechnology, 2025, 15(4): 606-614.

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免疫磁分离技术在循环肿瘤细胞检测中的研究进展

Research Progress of Immunomagnetic Separation Technology in Detection of Circulating Tumor Cell

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