关键词: 巴戟天, 环磷酰胺, 少弱精症, 氧化应激损伤, 精准发酵

Abstract:

The incidence of oligospermia and asthenozoospermia in China has been increasing year by year， but there is a lack of effective treatment methods. Therefore， the in-depth development of traditional Chinese medicine has become a research focus. Explored the improvement effect and potential mechanism of precise fermentation of Morinda officinalis by Lactobacillus fermentum Y5 on cyclophosphamide （CTX）?-induced oligoasthenospermia model in mice. Randomly divide the test mice into four groups： control group， model group， unfermented group， and fermented group. Use a sperm analyzer to detect the number and vitality of sperm in the mouse epididymis； Hematoxylin-eosin staining（HE） was performed on mouse testes， and the levels of reactive oxygen species （ROS） and malondialdehyde （MDA） were measured by ELISA. Western blot was used to detect the expression of Keap1/Nrf2/HO-1 signaling pathway proteins in mouse testes. The results showed that compared with the model group， the germ cells in the testes of mice in the fermented and unfermented groups were restored， and the number of sperm in the lumen increased； the sperm count and vitality of mice were significantly improved （P<0.01）； the levels of ROS and MDA in testicular tissue decreased （P<0.01）； Keap1 protein expression decreased， while Nrf2 and HO-1 protein expression increased （P<0.01）； compared with the unfermented group， the improvement effect of the fermented group was more significant （P<0.05）. The results showed that fermented Morinda officinalis can reduce oxidative stress damage and alleviate oligozoospermia in mice through the Keap1/Nrf2/HO-1 signaling pathway， and the improvement effect of precision fermented Morinda officinalis was better than that of unfermented Morinda officinalis.

Key words: Morinda officinalis, cyclophosphamide, oligoasthenospermia, oxidative stress damage, fermentation processing