冷链食品中铜绿假单胞菌的检测方法研究及进化树构建

doi:10.19586/j.2095-2341.2023.0132

生物技术进展 ›› 2023, Vol. 13 ›› Issue (6): 940-944.DOI: 10.19586/j.2095-2341.2023.0132

• 研究论文 • 上一篇

冷链食品中铜绿假单胞菌的检测方法研究及进化树构建

柯振华()

福建省产品质量检验研究院，国家加工食品质量监督检验中心（福州），福州 350000

收稿日期:2023-10-23 接受日期:2023-11-28 出版日期:2023-11-25 发布日期:2023-12-12
作者简介:柯振华 E-mail: iibio@163.com
基金资助:
福建省科技计划项目(2021Y0058)

Research on Detection Methods of Pseudomonas aeruginosa in Cold Chain Food and Construction of Evolutionary Tree

Zhenhua KE()

National Centre for Quality Supervision and Testing of Processed Food （Fuzhou），Fujian Inspection and Research Institute for Product Quality，Fuzhou 350000，China

Received:2023-10-23 Accepted:2023-11-28 Online:2023-11-25 Published:2023-12-12

摘要/Abstract

摘要：

铜绿假单胞菌是条件致病菌，对于患者具有较大的感染风险与危害，因此建立冷链食品中铜绿假单胞菌的检测方法并完成其溯源分子进化树构建工作，对于保障食品安全至关重要。以冷链食品中的铜绿假单胞菌为研究对象，应用实时荧光PCR检测技术建立快速检测方法。通过冷链食品抽样调查检测出铜绿假单胞菌后，将样品中检测出的铜绿假单胞菌菌株进行测序分析并建立铜绿假单胞菌生物进化树完成溯源分析。结果利用该方法成功从随机采集的271份冷链食品中检出10株病原菌，病原菌总体检出率为3.69%(10/271)，并通过分子进化树的构建成功溯源铜绿假单胞菌的污染来源并完成菌种种属定位。研究成果可以为冷链食品中铜绿假单胞菌等相关病原菌的检测溯源分析提供思路与方法。

关键词: 冷链食品, 铜绿假单胞菌, 生物进化树, 实时荧光PCR检测

Abstract:

Pseudomonas aeruginosa is a conditionally pathogenic bacterium with greater infection risk and harm to patients. Establishing a detection method for P. aeruginosa in cold chain foods and completing the construction of a traceable molecular evolutionary tree is crucial for food safety. Real-time fluorescence PCR technology was used to establish a rapid detection method for P. aeruginosa in cold chain food. After the detection of P. aeruginosa through cold chain food sampling investigation， the strains detected in the samples were sequenced and analyzed， and a biological evolutionary tree of P. aeruginosa was established to complete the traceability analysis. As a result， a total of 10 pathogenic bacteria were successfully detected from 271 randomly collected cold chain foods using this method， and the overall detection rate of pathogenic bacteria was 3.69% （10/271）. The source of contamination of P. aeruginosa was successfully traced and the localization of various genera was completed. The research results could provide ideas and methods for the detection and traceability analysis of P. aeruginosa and other related pathogens in cold chain food.

Key words: cold chain food, Pseudomonas aeruginosa, biological evolutionary tree, real-time fluorescence PCR

中图分类号:

Q93-31

柯振华. 冷链食品中铜绿假单胞菌的检测方法研究及进化树构建[J]. 生物技术进展, 2023, 13(6): 940-944.

Zhenhua KE. Research on Detection Methods of Pseudomonas aeruginosa in Cold Chain Food and Construction of Evolutionary Tree[J]. Current Biotechnology, 2023, 13(6): 940-944.

图/表 4

参考文献 26

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假单胞菌属菌株序列信息	中文名称
>NR_119225.1 Pseudomonas multiresinivorans strain ATCC 700690 16S ribosomal RNA, partial sequence	多树脂假单胞菌
>NR_179700.1 Pseudomonas tianjinensis strain 68 16S ribosomal RNA, partial sequence	天津假单胞菌
>NR_125523.1 Pseudomonas chengduensis strain MBR 16S ribosomal RNA, partial sequence	成端假单胞菌
>NR_114194.1 Pseudomonas citronellolis strain NBRC 103043 16S ribosomal RNA, partial sequence	香茅假单胞菌
>NR_026533.1 Pseudomonas citronellolis strain DSM 50332 16S ribosomal RNA, partial sequence	香茅假单胞菌
>NR_042435.1 Pseudomonas nitroreducens strain IAM 1439 16S ribosomal RNA, partial sequence	硝基还原假单胞菌
>NR_181536.1 Pseudomonas phenolilytica strain RBPA9 16S ribosomal RNA, partial sequence	溶酚假单胞菌
>NR_180597.1 Pseudomonas nicosulfuronedens strain LAM1902 16S ribosomal RNA, partial sequence	烟嘧磺酮假单胞菌
>NR_179202.1 Pseudomonas furukawaii strain KF707 16S ribosomal RNA, partial sequence	古河假单胞菌
>NR_181770.1 Pseudomonas boanensis strain DB1 16S ribosomal RNA, partial sequence	博阿南假单胞菌
>NR_179771.1 Pseudomonas lalkuanensis strain PE08 16S ribosomal RNA, partial sequence	拉尔夸南假单胞菌
>NR_117678.1 Pseudomonas aeruginosa strain DSM 50071 16S ribosomal RNA, partial sequence	铜绿假单胞菌

假单胞菌属菌株序列信息	中文名称
>NR_119225.1 Pseudomonas multiresinivorans strain ATCC 700690 16S ribosomal RNA, partial sequence	多树脂假单胞菌
>NR_179700.1 Pseudomonas tianjinensis strain 68 16S ribosomal RNA, partial sequence	天津假单胞菌
>NR_125523.1 Pseudomonas chengduensis strain MBR 16S ribosomal RNA, partial sequence	成端假单胞菌
>NR_114194.1 Pseudomonas citronellolis strain NBRC 103043 16S ribosomal RNA, partial sequence	香茅假单胞菌
>NR_026533.1 Pseudomonas citronellolis strain DSM 50332 16S ribosomal RNA, partial sequence	香茅假单胞菌
>NR_042435.1 Pseudomonas nitroreducens strain IAM 1439 16S ribosomal RNA, partial sequence	硝基还原假单胞菌
>NR_181536.1 Pseudomonas phenolilytica strain RBPA9 16S ribosomal RNA, partial sequence	溶酚假单胞菌
>NR_180597.1 Pseudomonas nicosulfuronedens strain LAM1902 16S ribosomal RNA, partial sequence	烟嘧磺酮假单胞菌
>NR_179202.1 Pseudomonas furukawaii strain KF707 16S ribosomal RNA, partial sequence	古河假单胞菌
>NR_181770.1 Pseudomonas boanensis strain DB1 16S ribosomal RNA, partial sequence	博阿南假单胞菌
>NR_179771.1 Pseudomonas lalkuanensis strain PE08 16S ribosomal RNA, partial sequence	拉尔夸南假单胞菌
>NR_117678.1 Pseudomonas aeruginosa strain DSM 50071 16S ribosomal RNA, partial sequence	铜绿假单胞菌

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冷链食品中铜绿假单胞菌的检测方法研究及进化树构建

Research on Detection Methods of Pseudomonas aeruginosa in Cold Chain Food and Construction of Evolutionary Tree

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