关键词: 北柴胡, ISSR, PCR扩增, 聚类分析, 遗传多样性

Abstract:

The genetic diversity of 10 different populations of Bupleurum chinense was studied by inter-simple sequence repeat （ISSR） molecular marker technique， in order to provide basis for further breeding of B. chinense. The DNA of Bupleurum chinense samples from fresh leaves of different populations was extracted by the plant tissue DNA extraction kit. The single factor gradient experiment was used to study the optimal ISSR-PCR reaction system. The DNA amplification products of different populations were subjected to agarose gel electrophoresis， and the genetic diversity of different populations of B. chinense was analyzed by NTSYS-pc software. The results showed that the genetic similarity coefficient of 10 populations ranged from 0.142 8 to 1.000 0. 10 different populations were divided into three categories by cluster analysis， with the first category being Gansu. The second category included five samples of B. chinense， Lingchuan， Henan， Wenxi， Pinglu， and Xiangfen， all belonging to the Jinnan population in Shanxi Province. The third category included four samples， namely Inner Mongolia， Linfen， Hunyuan， and Guangling， belonging to the northern population of Shanxi Province and Inner Mongolia. The 10 populations of B. chinense have relatively abundant genetic diversity and are related to their geographical environment. The results could provide theoretical basis for germplasm resources protection and elite germplasm breeding of B. chinense.

Key words: Bupleurum chinense, ISSR, PCR amplification, cluster analysis, genetic diversity