三烯生育酚研究进展

doi:10.19586/j.2095-2341.2021.0167

生物技术进展 ›› 2021, Vol. 11 ›› Issue (6): 668-675.DOI: 10.19586/j.2095-2341.2021.0167

三烯生育酚研究进展

李彦娇¹(), 高媛¹, 王磊¹^,²(), 张兰¹^,²()

^1.中国农业科学院生物技术研究所，北京 100081
^2.三亚中国农业科学院南繁研究院，海南三亚 572000

收稿日期:2021-10-11 接受日期:2021-10-28 出版日期:2021-11-25 发布日期:2021-11-26
通讯作者: 王磊,张兰
作者简介:李彦娇 E-mail： 1217558152@qq.com
基金资助:
国家转基因生物新品种培育重大专项(2016ZX08003-002);三亚崖州湾科技城管理局2020年度科技计划项目(SKJC- 2020-02-005)

Research Progress of Tocotrienol

Yanjiao LI¹(), Yuan GAO¹, Lei WANG¹^,²(), Lan ZHANG¹^,²()

^1.Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China
^2.National Nanfan Research Institute （Sanya），Chinese Academy of Agricultural Sciences，Hainan Sanya 572000，China

Received:2021-10-11 Accepted:2021-10-28 Online:2021-11-25 Published:2021-11-26
Contact: Lei WANG,Lan ZHANG

摘要/Abstract

摘要：

三烯生育酚和生育酚统称为维生素E，是重要的脂溶性维生素。维生素E只能在植物或者光合细菌中合成，是人类和动物必需且只能通过食物等摄取的重要维生素。一直以来，由于三烯生育酚与生育酚相比，生物活性较低且分布范围较小，人们对其研究相对较少。近些年的研究发现，由于三烯生育酚和生育酚的结构相似，因此三烯生育酚具有与生育酚相同的抗氧化等功能；但又由于三烯生育酚含有不饱和的植基侧链，使得三烯生育酚还具有一些不同于生育酚的功能，比如保护神经免受损伤、降低胆固醇、保护脑细胞免受损伤等。因此，三烯生育酚逐渐成为了研究热点。根据维生素E的生物合成途径，人们也开始了对三烯生育酚的生物强化研究，其合成途径中第一个关键酶基因HGGT的过表达是目前三烯生育酚含量提高的最有效途径；将来还需结合其合成调控的分子机制及其吸收利用问题，开发针对三烯生育酚的功能型产品。从三烯生育酚的合成途径、生物学功能、生物强化等方面进行了综述，并对今后的研究重点提出了展望。

关键词: 三烯生育酚, 合成途径, 生物学功能, 生物强化

Abstract:

Tocotrienol and tocopherol collectively referred to as vitamin E， are important fat?soluble vitamins. Vitamin E can only be synthesized by plants or photosynthetic bacteria. It is essential for human and animal nutrition and can only be ingested from outside. For a long time， due to its lower bioactivity and limited distribution than tocopherol， few research has been done on tocotrienol. Recent studies have found that due to the similar structure of tocotrienol and tocopherol， tocotrienol has the same antioxidant as tocopherol. While due to the unsaturated side chain， tocotrienol also has some functions different from tocopherol， such as protecting nerves from damage， lowering cholesterol and protecting brain cells from damage. Therefore， the research of tocotrienol has gradually become a hot spot. According to the biosynthetic pathway of vitamin E， people have also begun the study of tocotrienol biofortification， overexpression of the first key enzyme gene HGGT is the most effective way to improve the content of tocotrienol. In the future， it is necessary to combine the molecular mechanism of its synthesis and regulation and its absorption and utilization to develop functional products targeting at tocotrienol. In this paper， the synthetic pathway， biological function and biofortification of tocotrienol were reviewed， and the research focus in the future was prospected.

Key words: tocotrienol, biosynthetic pathway, biological function, biofortification

中图分类号:

Q566

李彦娇, 高媛, 王磊, 张兰. 三烯生育酚研究进展[J]. 生物技术进展, 2021, 11(6): 668-675.

Yanjiao LI, Yuan GAO, Lei WANG, Lan ZHANG. Research Progress of Tocotrienol[J]. Current Biotechnology, 2021, 11(6): 668-675.

图/表 1

参考文献 61

1	EVANS H M, BISHOP K S. On the existence fo a hitherto unrecognized dietary factor essential for reproduction [J]. Science, 1922, 56(1458): 650-651.
2	VALENTIN H E, QI Q. Biotechnological production and application of vitamin E: current state and prospects[J]. Appl. Microbiol. Biotechnol., 2005. 68(4): 436-444.
3	张兰,王磊. 植物中维生素E的生物学功能研究进展[J]. 生物技术进展,2016,6(6):389-395.
4	BABURA S R, NOR S, ABDULLAH A, et al.. Advances in genetic improvement for tocotrienol production: a review[J]. J. Nutr. Sci. Vitaminol., 2017, 63(4):215-221.
5	姚兴兰,王磊,张兰. 植物维生素E生物强化研究进展[J]. 生物技术进展, 2020,10(05):45-52.
6	SALIMATH S S, ROMSDAHL T B, KONDA A R, et al.. Production of tocotrienols in seeds of cotton (Gossypium hirsutum L.) enhances oxidative stability and offers nutraceutical potential[J]. Plant Biotechnol. J., 2021, 19(6):1268-1282.
7	SEN C K, KHANNA S, ROY S. Tocotrienols: vitamin E beyond tocopherols[J]. Life Sci., 2006, 78(18):2088-2098.
8	AGGARWAL B B, SUNDARAM C, PRASAD S, et al.. Tocotrienols, the vitamin E of the 21st century: its potential against cancer and other chronic diseases[J]. Biochem. Pharmacol., 2010, 80(11):1613-1631.
9	HORVATH G, WESSJOHANN L, BIGIRIMANA J, et al.. Differential distribution of tocopherols and tocotrienols in photosynthetic and non-photosynthetic tissues[J]. Phytochemistry, 2006, 67(12):1185-1195.
10	FALK J, MUNNÉ-BOSCH S. Tocochromanol functions in plants: antioxidation and beyond[J]. J. Exp. Bot., 2010, 61(6):1549-1566.
11	MUNNE-BOSCH S, ALEGRE L. The function of tocopherols and tocotrienols in plants[J]. Crit. Rev. Plant Sci., 2002, 21(1):31-57.
12	CAHOON E B, HALL S E, RIPP K G, et al.. Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content[J]. Nat. Biotechnol., 2003, 21(9):1082-1087.
13	ZENG Z, HAN N, LIU C, et al.. Functional dissection of HGGT and HPT in barley vitamin E biosynthesis via CRISPR/Cas9-enabled genome editing[J]. Ann. Bot., 2020. 126(5):929-942.
14	HU, X, LIU J, LI W, et al.. Biosynthesis and accumulation of multi-vitamins in black sweet corn (Zea mays L.) during kernel development[J]. J. Sci. Food Agric., 2020, 100(14):5230-5238.
15	ZHANG G Y, LIU R R, XU G, et al.. Increased α-tocotrienol content in seeds of transgenic rice overexpressing Arabidopsis γ-tocopherol methyltransferase[J]. Transgenic Res., 2013, 22(1):89-99.
16	HASSANIEN M M M, ABDEL-RAZEK A G, RUDZINSKA M, et al.. Phytochemical contents and oxidative stability of oils from non-traditional sources[J]. Eur. J. Lipid Sci. Technol., 2014, 116(11):1563-1571.
17	WONG R S Y, RADHAKRISHNAN A K. Tocotrienol research: past into present[J]. Nutr. Rev., 2012, 70(9):483-490.
18	SUZUKI Y J, TSUCHIYA M, WASSALL S R, et al.. Structural and dynamic membrane properties of alpha-tocopherol and alpha-tocotrienol: implication to the molecular mechanism of their antioxidant potency[J]. Biochemistry, 1993, 32(40):10692-10699.
19	KHOSLA P, PATEL V, WHINTER J M, et al.. Postprandial levels of the natural vitamin E tocotrienol in human circulation[J]. Antiox. Redox Sign., 2006, 8(5-6):1059-1068.
20	MATRINGE M, KSAS B, REY P, et al.. Tocotrienols, the unsaturated forms of vitamin E, can function as antioxidants and lipid protectors in tobacco leaves[J]. Plant Physiol., 2008, 147(2):764-778.
21	CHE P, ZHAO Z Y, GLASSMAN K, et al.. Elevated vitamin E content improves all-trans beta-carotene accumulation and stability in biofortified sorghum[J]. Proc. Natl. Acad. Sci. USA, 2016, 113(39):11040-11045.
22	STEINBERG D, PARTHASARATHY S, CAREW T E, et al.. Beyond cholesterol. modifications of low-density lipoprotein that increase its atherogenicity[J]. New Eng. J. Med., 1989, 320(14):915-924.
23	VALENZA M, SERBINOVA E, PACKER L, et al.. Nitecapone protects the Langendorff perfused heart against ischemia-reperfusion injury[J]. Biochem. Mol. Biol. Intern., 1993, 29(3):443-449.
24	AGGARWAL B B. Inflammation, a silent killer in cancer is not so silent! [J]. Curr. Opin. Pharmacol., 2009, 9(4):347-350.
25	GRIVENNIKOV S I, GRETEN F R, Immunity KARIN M., inflammation, and cancer[J]. Cell, 2010, 140(6):883-899.
26	PRASAD K. Tocotrienols and cardiovascular health[J]. Curr. Pharm. Des., 2011, 17(21):2147-2154.
27	WU S J, LIU P L, NG L T. Tocotrienol-rich fraction of palm oil exhibits anti-inflammatory property by suppressing the expression of inflammatory mediators in human monocytic cells[J]. Mol. Nutr. Food Res., 2008, 52(8):921-929.
28	AHN K S, SETHI G, KRISHNAN K, et al.. Gamma-tocotrienol inhibits nuclear factor-kappaB signaling pathway through inhibition of receptor-interacting protein and TAK1 leading to suppression of antiapoptotic gene products and potentiation of apoptosis[J]. J. Biol. Chem., 2007, 282(1):809-820.
29	SYLVESTER P W, AYOUB N M. Tocotrienols target PI3K/Akt signaling in anti-breast cancer therapy[J]. Antican. Agents Med. Chem., 2013, 13(7):1039-1047.
30	WEI L H, KUO M L, CHEN C A, et al.. The anti-apoptotic role of interleukin-6 in human cervical cancer is mediated by up-regulation of Mcl-1 through a PI 3-K/Akt pathway[J]. Oncogene, 2001, 20(41):5799-5809.
31	HUSAIN K, FRANCOIS R A, YAMAUCHI T, et al.. Vitamin E δ-tocotrienol augments the antitumor activity of gemcitabine and suppresses constitutive NF-κB activation in pancreatic cancer[J]. Mol. Cancer Ther., 2011, 10(12):2363-2372.
32	REN Z, PAE M, DAO M C, et al.. Dietary supplementation with tocotrienols enhances immune function in C57BL/6 mice[J]. J. Nutr., 2010, 140(7):1335-1341.
33	MAHALINGAM D, RADHAKRISHNAN A K, AMOM Z, et al.. Effects of supplementation with tocotrienol-rich fraction on immune response to tetanus toxoid immunization in normal healthy volunteers[J]. Eur. J. Clin. Nutr., 2011, 65(1):63-69.
34	ZHAO L, YAGIZ Y, XU C, et al.. Muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation[J]. Food Funct., 2015, 6(7):2293-2302.
35	KHANNA S, ROY S, PARINANDI N L, et al.. Characterization of the potent neuroprotective properties of the natural vitamin E alpha-tocotrienol[J]. J. Neurochem., 2006, 98(5):1474-1486.
36	SEN C K, KHANNA S, ROY S, et al.. Molecular basis of vitamin E action. Tocotrienol potently inhibits glutamate-induced pp60(c-Src) kinase activation and death of HT4 neuronal cells[J]. J. Biol. Chem., 2000, 275(17):13049-13055.
37	KHANNA S, ROY S, RYU H, et al.. Molecular basis of vitamin E action: tocotrienol modulates 12-lipoxygenase, a key mediator of glutamate-induced neurodegeneration[J]. J. Biol. Chem., 2003, 278(44):43508-43515.
38	SEN C K, KHANNA S, ROY S. Tocotrienols in health and disease: the other half of the natural vitamin E family[J]. Mol. Aspects Med., 2007, 28(5-6):692-728.
39	KHANNA S, PARINANDI N L, KOTHA S R, et al.. Nanomolar vitamin E alpha-tocotrienol inhibits glutamate-induced activation of phospholipase A2 and causes neuroprotection[J]. J. Neurochem., 2010, 112(5):1249-1260.
40	OSAKADA F, HASHINO A, KUME T, et al.. Alpha-tocotrienol provides the most potent neuroprotection among vitamin E analogs on cultured striatal neurons[J]. Neuropharmacology, 2004, 47(6):904-915.
41	QURESHI A A, SAMI S A, SALSER W A, et al.. Synergistic effect of tocotrienol-rich fraction [TRF(25)] of rice bran and lovastatin on lipid parameters in hypercholesterolemic humans[J]. J. Nutr. Biochem., 2001, 12(6):318-329.
42	QURESHI A A, SAMI S A, SALSER W A, et al.. Dose-dependent suppression of serum cholesterol by tocotrienol-rich fraction (TRF25) of rice bran in hypercholesterolemic humans[J]. Atherosclerosis, 2002, 161(1):199-207.
43	CICERO A F, GADDI A. Rice bran oil and gamma-oryzanol in the treatment of hyperlipoproteinaemias and other conditions[J]. Phytother. Res., 2001, 15(4):277-289.
44	PARKER R A, PEARCE B C, CLARK R W, et al.. Tocotrienols regulate cholesterol production in mammalian cells by post-transcriptional suppression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase[J]. J. Biol. Chem., 1993, 268(15):11230-11238.
45	TANAKA, H, YABUTA Y, TAMOI M, et al.. Generation of transgenic tobacco plants with enhanced tocotrienol levels through the ectopic expression of rice homogentisate geranylgeranyl transferase[J]. Plant Biotechnol., 2015, 32(3):233-238.
46	KONDA A R, NAZARENUS T J, NGUYEN H, et al.. Metabolic engineering of soybean seeds for enhanced vitamin E tocochromanol content and effects on oil antioxidant properties in polyunsaturated fatty acid-rich germplasm[J]. Metab. Eng., 2020, 57:63-73.
47	KIM Y H, LEE Y Y, KIM Y H, et al.. Antioxidant activity and inhibition of lipid peroxidation in germinating seeds of transgenic soybean expressing OsHGGT[J]. J. Agric. Food Chem., 2011, 59(2):584-591.
48	KARUNANANDAA B, QI Q, HAO M, et al.. Metabolically engineered oilseed crops with enhanced seed tocopherol[J]. Metab. Engin., 2005, 7(5-6):384-400.
49	RIPPERT P, SCIMEMI C, DUBALD M, et al.. Engineering plant shikimate pathway for production of tocotrienol and improving herbicide resistance[J]. Plant Physiol., 2004, 134(1):92-100.
50	ZHANG C, CAHOON R E, HUNTER S C, et al.. Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production[J]. Plant J., 2013, 73(4):628-639.
51	ESTÉVEZ J M, CANTERO A, REINDL A, et al.. 1-Deoxy-D-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants[J]. J. Biol. Chem., 2001, 276(25):22901-22909.
52	WRIGHT L P, ROHWER J M, GHIRARDO A,et al.. Deoxyxylulose 5-phosphate synthase controls flux through the methylerythritol 4-phosphate pathway in Arabidopsis[J]. Plant Physiol., 2014, 165(4):1488-1504.
53	CARRETERO-PAULET L, CAIRÓ A, BOTELLA-PAVÍA P, et al.. Enhanced flux through the methylerythritol 4-phosphate pathway in Arabidopsis plants overexpressing deoxyxylulose 5-phosphate reductoisomerase[J]. Plant Mol. Biol., 2006, 62(4-5):683-695.
54	RUIZ-SOLA M Á, COMAN D, BECK G, et al.. Arabidopsis GERANYLGERANYL DIPHOSPHATE SYNTHASE 11 is a hub isozyme required for the production of most photosynthesis-related isoprenoids[J]. New Phytol., 2016, 209(1):252-264.
55	姚兴兰,杨文竹,罗彦忠等, 转phyA2,ZmTMT和Bar玉米的获得及其特性分析[J]. 中国农业科学, 2020, 53(24):75-84.
56	MUNUSAMY U, NOR S, ABDULLAH A, et al.. Metabolic engineering of α-tocotrienol through PTGS mechanisms and isoprenoid/non-mevalonate pathways in perennial crops[J]. Plant Cell Biotechnol. Mol. Biol., 2015,16(3&4):119-129.
57	DOLDE D, WANG T. Oxidation of crude corn oil with and without elevated tocotrienols[J]. J. Am. Oil Chem. Soc., 2011, 88(9):1367-1372.
58	DIEPENBROCK C H, KANDIANIS C B, LIPKA A E, et al.. Novel loci underlie natural variation in vitamin E levels in maize grain[J]. Plant Cell, 2017, 29(10):2374-2392.
59	SUN T, ZHU Q, WEI Z, et al.. Multi-strategy engineering greatly enhances provitamin A carotenoid accumulation and stability in Arabidopsis seeds[J]. aBIOTECH, 2021,2(3):191-214.
60	CORDOBA E, SALMI M, LEÓN P. Unravelling the regulatory mechanisms that modulate the MEP pathway in higher plants[J]. J. Exp. Bot., 2009, 60(10):2933-2943.
61	KONG S L, ABDULLAH S N A, HO C L, et al.. Molecular cloning, gene expression profiling and in silico sequence analysis of vitamin E biosynthetic genes from the oil palm[J]. Plant Gene, 2016, 5:100-108.

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三烯生育酚研究进展

Research Progress of Tocotrienol

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