关键词: 五倍子, 白念珠菌, 差异蛋白, 同位素标记相对和绝对定量, 质谱

Abstract: In order to explore antifungal mechanism of Galla Chinensis against Candida albicans, the total proteins of Candida albicans (SC5314) treated with 4 μg/mL Galla Chinensis extract were extracted, and isobaric tags for relative and absolute quantization (iTRAQ) labeling and LC-MS/MS were used to analyse and identify differentially expressed proteins, and then the functions of the proteins were analyzed by bioinformatics. A total of 3 721 proteins were identified by LC-MS/MS. Among them, there were 104 differentially expressed proteins, with 57 proteins significantly up-regulated and 47 down-regulated. Through bioinformatics analysis, these differential proteins were involved in biological processes such as redox reactions, hydrogen peroxide catabolism, and energy metabolism process and so on. The results of this research indicated that the antifungal mechanism of Galla Chinensis against Candida albicans might be achieved by inhibiting oxidative phosphorylation, affecting cell energy metabolism and substance production, eventually resulting in changes in cell structure and function.

Key words: Galla Chinensis, Candida albicans, differential protein, isobaric tags for relative and absolute quantitation, mass spectrum