关键词: 金银花, 掺伪鉴定, 焦磷酸测序分析技术

Abstract: Honeysuckle is a staple medicine, adulteration in the market occurs sometimes, but the present detection method could not meet the requirements, therefore, it is urgent to establish a fast and accurate detection method. Combined with SNP labeling and pyrosequencing typing analysis technology, PCR amplification and pyrosequencing analysis were performed on the different SNP sites of Flos lonicerae and its common counterfeits, and an accurate and high-throughput method was established to detect the adulteration amount of Flos lonicerae. Results showed that, the signature SNP sites of Flos lonicerae and its counterfeits were quantitatively analyzed by pyrosequencing technology, and the authenticity and adulteration amount of Flos lonicerae were identified. This method has the sensitivity of PCR technology and the accuracy of sequencing technology, and is characterized by good repeatability, high degree of automation and simple operation. It can complete the detection of the adulteration amount of honeysuckle components within three hours and obtain the quantitative results.

Key words: honeysuckle, adulteration detection, pyrosequencing technology