关键词: 酰胺, 酰胺酶, 生物降解

Abstract: In order to explore the microscopic degradation mechanism of amide degrading enzymes, we used molecular docking method to investigate the binding modes of amide to amidase. The theoretical models of their structures were obtained. According to the lowest score function principle, the results showed that the best conformation scoring function between RhAmidase and L-methioninamide were -86.741 9, re-rank scores were -76.022 4. And we used LPC/CSU Server to study the interactions between RhAmidase and L-methioninamide. Results showed that hydrophobic interaction was the strongest contacts in the complex. The amino acid residues ARG256 A, LEU353 A, TYR346 A, ARG225 A, THR218 A and PRO222 A were detected to play significant roles in catalytic processes.

Key words: amide, amidase, biodegradation