关键词: 抗体, 糖基化, 分析方法, 生物学功能, 晚期糖基化终末产物

Abstract: Glycation is an important protein modification that could potentially affect bioactivity and molecular stability of the rapeutic proteins such as monoclonal antibodies. Many studies suggested that elevated levels of glycated hemoglobin were closely related with cardiovascular disease and atherosclerosis. Human plasma proteins, such as albumin, globulin, fibrinogen and collagen may be glycosylated, and then forms AGEs. In this paper, we reviewed the root cause and the current analytical methods of glycation of the rapeutic antibodies. We also summarized the effect of glycation on biological function, which was expected to provided reference for research on development, optimizion and storage condition of the rapeutic antibodies.

Key words: antibody, glycation, analytical methods, bioactivity, advanced glycation end products