关键词: 秀丽隐杆线虫, 铜绿假单胞菌, 抗感染, 免疫衰老

Abstract: Caenorhabditis elegans is an important model organism to study aging. However, it lacks an index of immuno-aging. In this study, through establishing C. elegans-Pseudomonas aeruginosa infection model, the relationship between anti-infective immune ability and aging of C. elegans was studied, and an index of C. elegans immuno-aging was established. Firstly, infection model was established using P. aeruginosa to infect C. elegans. C. elegans in different degrees of aging were used in the infection model to investigate the relationship between survival time and degree of aging. The survival time after infection was used as an anti-infective immuno-aging index. The data of immuno-aging index was compared with the data of antioxidant capacity and the lifespan. The results indicated that with the aging of C. elegans (7 d, 14 d and 21 d old), the survival time of infected C. elegans decreased sequentially, which was consistent with the decline of their antioxidant capacity. Compared with wildtype, daf-2 mutation C. elegans with long lifespan exhibited longer survival time after infection, while daf-16 mutation C. elegans with short lifespan exhibited shorter survival time with infection. The results of anti-infective immuno-aging index were in accordance with the data of lifespan index. Tyrosine, a compound with anti-aging activity, prolonged the survival time of C. elegans after infection. Collectively, C. elegans-P. aeruginosa infection model can be used to evaluate the immuno-aging of C. elegans. The survival time of C. elegans after infection can be used as an index to indicate immuno-aging of C. elegans.

Key words: Caenorhabditis elegans, Pseudomonas aeruginosa, anti-infection, immuno-aging