关键词: 水稻, BRs, 突变体, 图位克隆

Abstract: Brassinosteroids (BRs) are a kind of essential hormones that play important roles in plant growth and development. In Arabidopsis, many components of the BRs signaling pathway have been identified and studied in detail. However, some key components functioning in BRs signaling have not been described in rice. In this study, we identified an erect leave mutant from our rice mutant library, designated as el (erect leave mutant). Compared with wild-type plants, el exhibited erect leaves. Further physiological experiments showed that el is insensitive to exogenous BRs. Genetic analysis showed that the mutant is controlled by a dominant gene. The map-based cloning results showed that the mutant locus was located on rice chromosome 5 between primers InDel3 and InDel4, the physical distance is about 700 kb. In this study, we identified a new BRs insensitive mutant in rice, which will be an important material to uncover a new component in rice BRs signaling.

Key words: Oryza sativa, BRs, mutant, map-based cloning