关键词: LcChi2, 单子叶植物, 转基因水稻, 抗病性

Abstract: A new chitinase gene LcChi2 from Leymus chinensis showed enhanced resistance to fungal disease, when it was over-expressed in the dicotyledonous plant tobacco. Bioinformatics analysis suggested that LcChi2 encodes a type II chitinase classifying in the family 19. However, its resistance function to disease in the monocotyledon is unknown.  In this study, we took Jijing 88, a widely grown Japonica rice cultivar in Jilin province, as the receptor plant of which the LcChi2 and herbicide screening marker gene Bar were transferred via Agrobacterium-mediated transformation.  The transgenic rice at T1 stage was confirmed by PCR, RT-PCR and chitinase activity measurements, indicating that LcChi2 was integrated into the nuclear genome of rice and expressed effectively.  The transgenic rice was artificially inoculated with the rice blast pathogens, showing higher resistance to the blast. Also, the transgenic rice exhibited herbicide resistance suggesting that the herbicide resistant gene Bar is functioning.  Current results revealed that LcChi2 was able to improve the resistance to disease in the monocotyledons effectively, and might be used as a significant candidate disease-resistance gene for biotech breeding in the crops.

Key words: LcChi2, monocotyledon, transgenic rice, disease resistance