生物技术进展 ›› 2013, Vol. 3 ›› Issue (3): 157-161.DOI: 10.3969/j.issn.2095-2341.2013.03.01

• 进展评述 •    下一篇

植物选择标记基因剔除技术研究进展

苏月焱,王旭静,王志兴*   

  1. 中国农业科学院生物技术研究所, 北京 100081
  • 收稿日期:2013-03-19 出版日期:2013-05-25 发布日期:2013-04-23
  • 通讯作者: 王志兴,研究员,博士生导师,主要从事植物基因工程和转基因生物安全研究。E-mail:wangzhixing@caas.cn
  • 作者简介:苏月焱,硕士研究生,主要从事转基因生物安全研究。E-mail:alweou@126.com。
  • 基金资助:

    国家转基因生物新品种培育重大专项(2011ZX08010-003)资助。

Research Progress on Marker-free Plant Transgenic Technology

SU Yue-yan, WANG Xu-jing, WANG Zhi-xing   

  1. Biotechnology Research Institute,Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-03-19 Online:2013-05-25 Published:2013-04-23

摘要: 获得无选择标记基因的转基因作物是转基因作物发展的必然趋势。目前剔除选择标记基因的常用方法包括共转化、位点特异性重组、转座子、同源重组等。本文综述了这些常用技术的原理和目前的发展现状,同时对这些技术的特点进行描述,并对植物安全转基因技术发展进行展望,以期推动转基因作物的快速产业化。

关键词: 选择标记基因, 共转化, 位点特异性重组, 转座子, 同源重组

Abstract: Under the tendency rapid development of geneticallly modified orgnism(GMO), it is necessary to obtain the marker-free transgenic plants. At present,the marker-free transgenic technologies include co-transformation,site-specific recombination, transposons, homologous recombination and so on. This paper summarizes the character, the theories and research progress of marker-free and redundant sequence-free transgenic technologies, which are extensively used in recent research. Then, it also prospects the application potential of the safe transgenic technologies for the rapid commercialization of GMOs.

Key words: selectable marker gene, co-transformation, site-specific recombination; , transposons, homologous recombination