关键词: ku基因, 非同源末端连接(NHEJ), 同源重组频率

Abstract: Non-homologous end joining (NHEJ) mediated by ku genes is a repair type of DNA double-strand breaks (DSBs). NHEJ pathway is independent of homologous recombination (HR), and decreases HR with competitive relationship. ku genes are highly conservative in the biological evolution process, and ku mutants have been phenotypically characterized in many microorganisms, especially in fungi. Gene targeting frequency is increased by deletion of ku genes, when HR plays a key role on the defects of NHEJ pathway, leading to facilitate analysis of gene function.

Key words: ku gene, non-homologous end joining(NHEJ), homologous recombination frequency