碳纳米材料的细胞毒性及免疫效应研究进展

doi:10.19586/j.2095-2341.2025.0024

生物技术进展 ›› 2025, Vol. 15 ›› Issue (4): 615-621.DOI: 10.19586/j.2095-2341.2025.0024

• 进展评述 • 上一篇

碳纳米材料的细胞毒性及免疫效应研究进展

张怡淼(), 边裕钦(), 刘鑫博(), 庞佳禾, 孙同选, 杜洽政, 胥文豪, 尹天泽(), 隋宏书()

山东第一医科大学临床与基础医学院组织学与胚胎学系，济南 250117

收稿日期:2025-02-24 接受日期:2025-04-15 出版日期:2025-07-25 发布日期:2025-09-08
通讯作者: 尹天泽,隋宏书
作者简介:张怡淼 E-mail: 15137335275@163.com；
边裕钦 E-mail: 1664591559@qq.com
刘鑫博 E-mail: 3050404736@qq.com第一联系人：（张怡淼、边裕钦、刘鑫博并列第一作者）
基金资助:
国家自然科学基金项目(81670004);山东第一医科大学教育教学改革项目(XM2022046);山东第一医科大学大学生创新创业训练项目(202410439043)

Research Progress on the Cytotoxicity and Immune Effects of Carbon-based Nanomaterials

Yimiao ZHANG(), Yuqin BIAN(), Xinbo LIU(), Jiahe PANG, Tongxuan SUN, Qiazheng DU, Wenhao XU, Tianze YIN(), Hongshu SUI()

Department of Histology and Embryology，College of Clinical and Basic Medicine，Shandong First Medical University，Jinan 250117，China

Received:2025-02-24 Accepted:2025-04-15 Online:2025-07-25 Published:2025-09-08
Contact: Tianze YIN,Hongshu SUI

摘要/Abstract

摘要：

碳基纳米材料因其独特的物理化学性质在生物医学、能源存储及电子器件等领域展现出广阔的应用前景。近年来，碳纳米材料的毒理学研究取得了显著进展，但结构差异对细胞毒性及免疫效应的影响机制尚未完全明确。通过系统地分析现有文献资料，比较了多种碳纳米材料在体外细胞试验和体内动物模型中的表现，疏理了碳纳米材料的结构-毒性-免疫效应关联，总结了碳纳米材料毒理学研究的总体现状、发展趋势和研究需求，以期为其安全性设计提供理论依据。

关键词: 细胞毒性, 碳纳米材料, 巨噬细胞, 免疫效应

Abstract:

Carbon-based nanomaterials have demonstrated extensive application prospects in fields including biomedicine， energy storage， and electronic devices， owing to their unique physicochemical properties. In recent years， significant advancements have been achieved in toxicological studies of these materials， but the mechanisms through which structural variations influence their cytotoxicity and immune effects remain not yet fully elucidated. This review systematically synthesized existing literature， meticulously comparing the performance of diverse carbon nanomaterials in both in vitro cellular assays and in vivo animal models， sorted out the structure-toxicity-immune effect relationships of carbon nanomaterials， and summarized the current research landscapes， development trends and research needs of toxicology research on carbon nanomaterials， in order to provid theoretical basis for their safety design.

Key words: cytotoxicity, carbon nanomaterials, macrophage, immune effect

中图分类号:

张怡淼, 边裕钦, 刘鑫博, 庞佳禾, 孙同选, 杜洽政, 胥文豪, 尹天泽, 隋宏书. 碳纳米材料的细胞毒性及免疫效应研究进展[J]. 生物技术进展, 2025, 15(4): 615-621.

Yimiao ZHANG, Yuqin BIAN, Xinbo LIU, Jiahe PANG, Tongxuan SUN, Qiazheng DU, Wenhao XU, Tianze YIN, Hongshu SUI. Research Progress on the Cytotoxicity and Immune Effects of Carbon-based Nanomaterials[J]. Current Biotechnology, 2025, 15(4): 615-621.

参考文献 41

[1]	ALJABALI A A, OBEID M A, BASHATWAH R M, et al.. Nanomaterials and their impact on the immune system[J/OL]. Int. J. Mol. Sci., 2023, 24(3): 2008[2025-06-25]. .
[2]	KIM H Y, YOON J J, KIM D S, et al.. YG-1 extract improves acute pulmonary inflammation by inducing bronchodilation and inhibiting inflammatory cytokines[J/OL]. Nutrients, 2021, 13(10): 3414[2025-06-25]. .
[3]	DEVOY J, AL-ABED S, CERDAN B, et al.. Analysis of carbon nanotube levels in organic matter: an inter-laboratory comparison to determine best practice[J]. Nanotoxicology, 2024, 18(2): 214-228.
[4]	DÍEZ-PASCUAL A M. Carbon-based nanomaterials 4.0[J/OL]. Int. J. Mol. Sci., 2024, 25(5): 3032[2025-06-25]. .
[5]	KANDHOLA G, PARK S, WLIM J, et al.. Nanomaterial-based scaffolds for tissue engineering applications: a review on graphene, carbon nanotubes and nanocellulose[J]. Tissue Eng. Regen. Med., 2023, 20(3): 411-433.
[6]	YUAN X, ZHANG X, SUN L, et al.. Cellular toxicity and immunological effects of carbon-based nanomaterials[J/OL]. Part. Fibre Toxicol., 2019, 16(1): 18[2025-06-25]. .
[7]	LI Y, CAO J. The impact of multi-walled carbon nanotubes (MWCNTs) on macrophages: contribution of MWCNT characteristics[J]. Sci. China Life Sci., 2018, 61(11): 1333-1351.
[8]	NALETOVA I, TOMASELLO B, ATTANASIO F, et al.. Prospects for the use of metal-based nanoparticles as adjuvants for local cancer immunotherapy[J/OL]. Pharmaceutics, 2023, 15(5): 1346[2025-06-25]. .
[9]	PATRICK B, AKHTAR T, KOUSAR R, et al.. Carbon nanomaterials: emerging roles in immuno-oncology[J/OL]. Int. J. Mol. Sci., 2023, 24(7): 6600[2025-06-25]. .
[10]	BURRES C, WONG R, PEDREIRA F, et al.. A regulatory compliant short-term oral toxicity study of soluble [60] fullerenes in rats[J]. Excli. J., 2024, 23: 772-786.
[11]	CARY C, STAPLETON P. Determinants and mechanisms of inorganic nanoparticle translocation across mammalian biological barriers[J]. Arch. Toxicol., 2023, 97(8): 2111-2131.
[12]	FIGAROL A, POURCHEZ J, BOUDARD D, et al.. In vitro toxicity of carbon nanotubes, nano-graphite and carbon black, similar impacts of acid functionalization[J]. Toxicol. Vitro, 2015, 30(Pt B): 476-485.
[13]	KONG C, CHEN J, LI P, et al.. Respiratory toxicology of graphene-based nanomaterials: a review[J/OL]. Toxics, 2024, 12(1): 82[2025-06-25]. .
[14]	ZHANG M, XU Y, YANG M, et al.. Clearance of single-wall carbon nanotubes from the mouse lung: a quantitative evaluation[J]. Nanoscale Adv., 2020, 2(4): 1551-1559.
[15]	AWASHRA M, MYNARZ P. The toxicity of nanoparticles and their interaction with cells: an in vitro metabolomic perspective[J]. Nanoscale Adv., 2023, 5(10): 2674-2723.
[16]	WANG X, GUO J, CHEN T, et al.. Multi-walled carbon nanotubes induce apoptosis via mitochondrial pathway and scavenger receptor[J]. Toxicol. Vitro, 2012, 26(6): 799-806.
[17]	XU C, LIU Q, LIU H, et al.. Toxicological assessment of multi-walled carbon nanotubes in vitro: potential mitochondria effects on male reproductive cells[J]. Oncotarget, 2016, 7(26): 39270-39278.
[18]	KAGAN V E, KAPRALOV A A, CROIX C M, et al.. Lung macrophages "digest" carbon nanotubes using a superoxide/peroxynitrite oxidative pathway[J]. ACS Nano, 2014, 8(6): 5610-5621.
[19]	TIAN N, DUAN H, CAO T, et al.. Macrophage-targeted nanoparticles mediate synergistic photodynamic therapy and immunotherapy of tuberculosis[J]. RSC Adv., 2023, 13(3): 1727-1737.
[20]	CHETYRKINA M R, FEDOROV F S, NASIBULIN A G. In vitro toxicity of carbon nanotubes: a systematic review[J]. RSC Adv., 2022, 12(25): 16235-16256.
[21]	MIAO Y, WANG S, ZHANG B, et al.. Carbon dot-based nanomaterials: a promising future nano-platform for targeting tumor-associated macrophages[J/OL]. Front. Immunol., 2023, 14: 1133238[2025-06-25]. .
[22]	SALLAM A A, AHMED M M, EL-MAGD M A, et al.. Quercetin-ameliorated, multi-walled carbon nanotubes-induced immunotoxic, inflammatory, and oxidative effects in mice[J/OL]. Molecules, 2022, 27(7): 2117[2025-06-25]. .
[23]	WANG T H, WATANABE K, MUROMACHI K, et al.. Carbon nanotubes induce mineralization of human cementoblasts[J]. J. Endod., 2024, 50(8): 1117-1123.
[24]	SUN Z, WANG W, MENG J, et al.. Multi-walled carbon nanotubes conjugated to tumor protein enhance the uptake of tumor antigens by human dendritic cells in vitro [J]. Cell Res., 2010, 20(10): 1170-1173.
[25]	AHLAWAT J, ASIL S M, BARROSO G G, et al.. Application of carbon nano onions in the biomedical field: recent advances and challenges[J]. Biomater. Sci., 2021, 9(3): 626-644.
[26]	MEHRALIAN F, TADI BENI Y. Molecular dynamics analysis on axial buckling of functionalized carbon nanotubes in thermal environment[J/OL]. J. Mol. Model., 2017, 23(12): 330[2025-06-25]. .
[27]	LORENZO S, GONZÁLEZ-FERNÁNDEZ A. Handbook of immunological properties of engineered nanomaterials[M]. Singapore: World Scientific, 2013: 517-545.
[28]	FRIESEN A, FRITSCH-DECKER S, MÜLHOPT S, et al.. Comparing the toxicological responses of pulmonary air-liquid interface models upon exposure to differentially treated carbon fibers[J/OL]. Int. J. Mol. Sci., 2023, 24(3): 1927[2025-06-25]. .
[29]	SHEEMA A N, NAIKI-ITO A, KAKEHASHI A, et al.. Fullerene and fullerene whisker are not carcinogenic to the lungs and pleura in rat long-term study after 2-week intra-tracheal intrapulmonary administration[J]. Arch. Toxicol., 2024, 98(12): 4143-4158.
[30]	LIU K Y, GAO Y, XIAO W, et al.. Multidimensional analysis of lung lymph nodes in a mouse model of allergic lung inflammation following PM_2.5 and indeno [1, 2, 3-cd] pyrene exposure[J/OL]. Environ. Health Perspect., 2023, 131(3): 37014[2025-06-25]. .
[31]	PERVIZAJ-ORUQAJ L, SELVAKUMAR B, FERRERO M R, et al.. Alveolar macrophage-expressed Plet1 is a driver of lung epithelial repair after viral pneumonia[J/OL]. Nat. Commun., 2024, 15(1): 87[2025-06-25]. .
[32]	RONZANI C, CASSET A, PONS F. Exposure to multi-walled carbon nanotubes results in aggravation of airway inflammation and remodeling and in increased production of epithelium-derived innate cytokines in a mouse model of asthma[J]. Arch. Toxicol. 2014, 88(2):489-499.
[33]	MOHAMMED A N, YADAV N, KAUR P, et al.. Immunomodulation of susceptibility to pneumococcal pneumonia infection in mouse lungs exposed to carbon nanoparticles via dysregulation of innate and adaptive immune responses[J/OL]. Toxicol. Appl. Pharmacol., 2024, 483: 116820[2025-06-25]. .
[34]	RANJITKAR S, KRAJEWSKI D, TEDESCHI C, et al.. Mast cell responses in a mouse model of food allergy are regulated via a ST2/IL-4 axis[J]. Allergy, 2024, 79(9): 2561-2564.
[35]	WANG Q, HAN J, WEI M, et al.. Multi-walled carbon nanotubes accelerate leukaemia development in a mouse model[J/OL]. Toxics, 2024, 12(9): 646[2025-06-25]. .
[36]	KRUPNIK L, JOSHI P, KAPPLER A, et al.. Critical nanomaterial attributes of iron-carbohydrate nanoparticles: leveraging orthogonal methods to resolve the 3-dimensional structure[J/OL]. Eur. J. Pharm. Sci., 2023, 188: 106521[2025-06-25]. .
[37]	SHARMA M, ALESSANDRO P, CHERIYAMUNDATH S, et al.. Therapeutic and diagnostic applications of carbon nanotubes in cancer: recent advances and challenges[J]. J. Drug Target., 2024, 32(3): 287-299.
[38]	PANNUZZO M, ESPOSITO S, WU L P, et al.. Overcoming nanoparticle-mediated complement activation by surface PEG pairing[J]. Nano Lett., 2020, 20(6): 4312-4321.
[39]	ALJABALI A A, OBEID M A, BASHATWAH R M, et al.. Nanomaterials and their impact on the immune system[J/OL]. Int. J. Mol. Sci., 2023, 24(3): 2008[2025-06-25]. .
[40]	TAYLOR-JUST A J, IHRIE M D, DUKE K S, et al.. The pulmonary toxicity of carboxylated or aminated multi-walled carbon nanotubes in mice is determined by the prior purification method[J/OL]. Part. Fibre Toxicol., 2020, 17(1): 60[2025-06-25]. .
[41]	QING T L, JIANG X Y, LI J F, et al.. Celastrol reduces lung inflammation induced by multiwalled carbon nanotubes in mice via NF-κB-signaling pathway[J]. Inhal. Toxicol., 2024, 36(4): 275-281.

碳纳米材料的细胞毒性及免疫效应研究进展

Research Progress on the Cytotoxicity and Immune Effects of Carbon-based Nanomaterials

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 41

相关文章 6

编辑推荐

Metrics

本文评价

[1]	吕家硕, 任衍棋, 徐湘敏, 张译文, 刘晓晖, 刘成珍. 微塑料及其生物降解研究进展[J]. 生物技术进展, 2024, 14(5): 768-775.
[2]	段兴鹏, 刘景丽, 王澈, 尚德静. 巨噬细胞清道夫受体与Toll样受体对Ox-LDL摄取和炎症的影响[J]. 生物技术进展, 2024, 14(4): 668-675.
[3]	席照青, 包明威. 巨噬细胞糖脂代谢重编程在非酒精性脂肪肝中的研究进展[J]. 生物技术进展, 2024, 14(3): 399-405.
[4]	高丽, 杨磊, 李光鹏. Myostatin基因突变激发骨骼肌发育机制研究进展[J]. 生物技术进展, 2021, 11(4): 476-482.
[5]	YAO Mawulikplimi Adzavon,赵鹏翔,张旭娟,王丽敏,马雪梅. 巨噬细胞迁移抑制因子分子机制研究进展[J]. 生物技术进展, 2018, 8(5): 389-396.
[6]	路艳,陈莹,陆庆明,张颖,李朝晖,谢晓华 . 大鼠创伤失血性休克心肌巨噬细胞极化标志蛋白变化规律研究[J]. 生物技术进展, 2017, 7(3): 225-229.