关键词: 心肌, 创伤失血性休克, 巨噬细胞极化标志蛋白, 免疫组织化学

Abstract: In order to study on the change rule of myocardial macrophage polarization biomarker based on the rat traumatic hemorrhagic shock model, adult Wistar male rats were chosen, and randomly divided into control, 1 hour trauma, 2 hours trauma, 4 hours trauma, 8 hours trauma,16 hours trauma, 24 hours trauma and 48 hours trauma groups (ten rats per group),  and rat traumatic hemorrhagic shock model was established based on mechanical impact method followed with rats behavior recorded. After modeling, rats were killed, and myocardial tissue was collected to perform immunohistochemistry (IHC) on the M0, M1, M2-type macrophage biomarkers, including CD68, nitric oxide synthase (iNOS), and arginase-1 (ARG-1), respectively. Results showed that, CD68 expression level was significantly increased with modeling time prolonging, and reached to the peak value at 24 hours after modeling, and gradually decreased at 24 hours to 48 hours after modeling. Meanwhile, iNOS expression level was detected at 16 hours after modeling, and gradually increased at 16 hours to 48 hours after modeling. Similarly, the expression level of ARG-1 was detected at 16 hours, and rapidly increased at 16 hours to 48 hours. The results suggested that the rat traumatic hemorrhagic shock model was correctly established using mechanical impact method, and the expression level of macrophage polarization biomarkers were significantly changed with modeling time prolonging, and provided a significant reference for the study of myocardium traumatic hemorrhagic shock, and exhibited a certain application value in clinic.

Key words: myocardium, traumatic hemorrhagic shock, macrophage polarization biomarkers, immunohistochemistry