海洋链霉菌隐性次级代谢产物基因簇激活的研究进展

doi:10.19586/j.2095-2341.2024.0141

生物技术进展 ›› 2025, Vol. 15 ›› Issue (1): 19-26.DOI: 10.19586/j.2095-2341.2024.0141

海洋链霉菌隐性次级代谢产物基因簇激活的研究进展

王博轩(), 陈佳鑫, 蔡佳君, 李昆贤, 林家纯, 唐灵芝, 洪璇()

厦门医学院，厦门市海洋药用天然产物资源重点实验室，福建厦门 361023

收稿日期:2024-08-28 接受日期:2024-10-14 出版日期:2025-01-25 发布日期:2025-03-07
通讯作者: 洪璇
作者简介:王博轩 E-mail：1246884719@qq.com；
基金资助:
福建省大学生创新创业训练项目(202412631010);厦门市自然科学基金面上项目(3502Z20227227);厦门医学院校级科研项目(K2023-25);厦门医学院教育教学改革研究项目(XBKS2022002)

Research Progress on Metabolites of Marine Streptomyces Under the Strategy of Activating Silencing Genes

Boxuan WANG(), Jiaxin CHEN, Jiajun CAI, Kunxian LI, Jiachun LIN, Lingzhi TANG, Xuan HONG()

Xiamen Key Laboratory of Marine Medicinal Natural Products Resources，Xiamen Medical College，Fujian Xiamen 361023，China

Received:2024-08-28 Accepted:2024-10-14 Online:2025-01-25 Published:2025-03-07
Contact: Xuan HONG

摘要/Abstract

摘要：

海洋链霉菌具有复杂的发育分化周期，在进行形态分化的同时常伴随着复杂的生理变化，因此，海洋链霉菌是具有生物活性的先导化合物的重要来源。然而，海洋链霉菌次级代谢产物的大部分生物合成基因簇是沉默状态。综述了预测“沉默基因”表达潜力的方法，总结了激活“沉默基因”表达的手段，包括改变培养条件、化学诱导基因簇表达和生物介导等，以期为海洋链霉菌次级代谢产物的挖掘提供新的途径。

关键词: 海洋链霉菌, 沉默基因, 多效性方法

Abstract:

Marine Streptomyces are the source of the valuable lead compounds with medicinal biological activity due to their complex morphological differentiation cycle， which often accompanied by complex physiological changes. But most of the biosynthetic gene clusters of secondary metabolites in marine Streptomyces are “silent”. The article reviewed the methods for predicting the expression potential of “silent genes” and summarized the means of activating “silent genes”， including changing culture conditions， chemically inducing gene cluster expression and biological mediation， in order to provide new avenues for the mining of secondary metabolites in marine Streptomyces.

Key words: marine Streptomyces, silent genes, multi efficiency method

中图分类号:

S917.1

王博轩, 陈佳鑫, 蔡佳君, 李昆贤, 林家纯, 唐灵芝, 洪璇. 海洋链霉菌隐性次级代谢产物基因簇激活的研究进展[J]. 生物技术进展, 2025, 15(1): 19-26.

Boxuan WANG, Jiaxin CHEN, Jiajun CAI, Kunxian LI, Jiachun LIN, Lingzhi TANG, Xuan HONG. Research Progress on Metabolites of Marine Streptomyces Under the Strategy of Activating Silencing Genes[J]. Current Biotechnology, 2025, 15(1): 19-26.

图/表 2

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来源	方法	化合物	生物活性	参考文献
Streptomyces sp. IMB18-531	共培养（Cladosporium sp. IMB19-099）	铝草氨菌素E、去铁胺E、铁草氨菌素E、Terragine E、卡巴西霉素、环 (L-脯氨酰-L-酪氨酸)、邻氨基苯甲酸、(Z)-14-甲基十五烷-9-烯酸和(Z)-十六烷-8-烯酸	去铁胺E显示出抗肝纤维化活性;卡巴西霉素、(Z)-14-甲基十五烷-9-烯酸和(Z)-十六烷-8-烯酸对甲氧西林耐药金葡菌、表皮葡萄球菌、枯草芽孢杆菌显出抗菌活性	[15]
Streptomyces sp. 891-B6	改变培养条件	金黄毒素A	用于治疗革兰氏阳性细菌感染和癌症	[16]
Streptomycessomaliensis SCSIO ZH66	核糖体工程	弗德利卡毒素	具有抗癌活性	[17]
Streptomyces sp. H-KF8	改变培养条件	诺卡胺素、麦角甾醇、星形孢菌素	诺卡胺素具有抑制病原菌的活性，麦角甾醇对多株肿瘤细胞株具有抑制毒性，星形孢菌素具有非常有效的蛋白激酶抑制活性	[18]
Streptomyces bullii	共培养( Aspergillus fumigatus MBC-F1-10)	Brevianamide、螺旋前列腺素 A、6-methoxy spirotryprostain B、烟曲霉震颤素B、烟曲霉震颤C、12，13-dihydroxy deriva tive、疣孢青霉原	均有灭杀寄生虫的活性	[19]
Streptomyces sp. S52-B	OSMAC策略	Ammosamide A、Ammosamide B、Ammosalic acid	化合物Ammosamide A和Ammosamide B具有优良的抗癌活性	[20]
Streptomyces sp. SCSIO T05	代谢工程	Lobophorin CR4	具有抗菌活性	[21]
Streptomyces koyangensis SCSIO 5802	基因组挖掘	10-epi-HSAF、Koyanamide A	10-epi-HSAF能够抑制多种卵菌和真菌活性	[22]
Streptomyces sp. SM17	基因组挖掘	Surugamide A	具有抗癌和抗真菌活性	[23]
Streptomyces sp. SUD119	化学诱导	Donghaecyclinones A-C	具有抗菌、抗真菌和抗癌活性	[24-26]

来源	方法	化合物	生物活性	参考文献
Streptomyces sp. IMB18-531	共培养（Cladosporium sp. IMB19-099）	铝草氨菌素E、去铁胺E、铁草氨菌素E、Terragine E、卡巴西霉素、环 (L-脯氨酰-L-酪氨酸)、邻氨基苯甲酸、(Z)-14-甲基十五烷-9-烯酸和(Z)-十六烷-8-烯酸	去铁胺E显示出抗肝纤维化活性;卡巴西霉素、(Z)-14-甲基十五烷-9-烯酸和(Z)-十六烷-8-烯酸对甲氧西林耐药金葡菌、表皮葡萄球菌、枯草芽孢杆菌显出抗菌活性	[15]
Streptomyces sp. 891-B6	改变培养条件	金黄毒素A	用于治疗革兰氏阳性细菌感染和癌症	[16]
Streptomycessomaliensis SCSIO ZH66	核糖体工程	弗德利卡毒素	具有抗癌活性	[17]
Streptomyces sp. H-KF8	改变培养条件	诺卡胺素、麦角甾醇、星形孢菌素	诺卡胺素具有抑制病原菌的活性，麦角甾醇对多株肿瘤细胞株具有抑制毒性，星形孢菌素具有非常有效的蛋白激酶抑制活性	[18]
Streptomyces bullii	共培养( Aspergillus fumigatus MBC-F1-10)	Brevianamide、螺旋前列腺素 A、6-methoxy spirotryprostain B、烟曲霉震颤素B、烟曲霉震颤C、12，13-dihydroxy deriva tive、疣孢青霉原	均有灭杀寄生虫的活性	[19]
Streptomyces sp. S52-B	OSMAC策略	Ammosamide A、Ammosamide B、Ammosalic acid	化合物Ammosamide A和Ammosamide B具有优良的抗癌活性	[20]
Streptomyces sp. SCSIO T05	代谢工程	Lobophorin CR4	具有抗菌活性	[21]
Streptomyces koyangensis SCSIO 5802	基因组挖掘	10-epi-HSAF、Koyanamide A	10-epi-HSAF能够抑制多种卵菌和真菌活性	[22]
Streptomyces sp. SM17	基因组挖掘	Surugamide A	具有抗癌和抗真菌活性	[23]
Streptomyces sp. SUD119	化学诱导	Donghaecyclinones A-C	具有抗菌、抗真菌和抗癌活性	[24-26]

海洋链霉菌隐性次级代谢产物基因簇激活的研究进展

Research Progress on Metabolites of Marine Streptomyces Under the Strategy of Activating Silencing Genes

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