根癌农杆菌介导的金针菇遗传转化研究进展

doi:10.19586/j.2095-2341.2024.0122

生物技术进展 ›› 2024, Vol. 14 ›› Issue (6): 902-910.DOI: 10.19586/j.2095-2341.2024.0122

• 食药用菌生物技术专题 • 上一篇下一篇

根癌农杆菌介导的金针菇遗传转化研究进展

唐桂容(), 刘建雨, 于海龙, 宋春艳, 谭琦, 尚晓冬()

上海市农业科学院食用菌研究所，国家食用菌工程技术研究中心，农业农村部应用真菌资源与利用重点实验室，上海 201403

收稿日期:2024-07-02 接受日期:2024-08-14 出版日期:2024-11-25 发布日期:2024-12-27
通讯作者: 尚晓冬
作者简介:唐桂容E-mail: grtang@saas.sh.cn；
基金资助:
国家自然科学基金项目(32202572)

Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens

Guirong TANG(), Jianyu LIU, Hailong YU, Chunyan SONG, Qi TAN, Xiaodong SHANG()

Key Laboratory of Edible Fungal Resources and Utilization （South），Ministry of Agriculture and Rural Affairs，National Engineering Research Center of Edible Fungi，Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，Shanghai 201403，China

Received:2024-07-02 Accepted:2024-08-14 Online:2024-11-25 Published:2024-12-27
Contact: Xiaodong SHANG

摘要/Abstract

摘要：

金针菇（Flammulina filiformis）是一种著名的药食两用真菌，具有良好的开发应用前景。高效稳定的遗传转化体系是其进行基因功能研究的关键技术。农杆菌介导的转基因方法是目前金针菇遗传转化的重要方法之一。系统综述了转化受体、农杆菌菌株、双元载体、乙酰丁香酮和共培养时间等因素对金针菇遗传转化效率的影响，总结了农杆菌介导的转化技术在金针菇基因功能研究中的应用进展，并就该技术存在的问题和应用前景作简单的探讨，以期为今后建立高效的农杆菌介导的金针菇遗传转化系统提供参考。

关键词: 金针菇, 农杆菌, 遗传转化

Abstract:

Flammulina filiformis， a famous medicinal and edible mushroom fungus in china， has a good prospect for development and application. An efficient and stable genetic transformation system is a key technology for studying its gene function. In currently， Agrobacterium-mediated gene transferring is one of the major methods in genetic transformation of F. filiformis. In this paper， key factors involved in Agrobacterium-mediated genetic transformation of F. filiformis incluing recipient tissue， Agrobacterium strains， binary vectors， acestosyringone， and co-culture time were systematically reviewed. The progress of Agrobacteria-mediated transformation technology in the study of gene function of F. filiformis was summarized， and the existing issues and application prospects of this technology were briefly discussed. The aim of this study is to provide a reference for the establishment of an efficient Agrobacterium-mediated genetic transformation system of F. filiformis for the future.

Key words: Flammulina filiformis, Agrobacterium tumefaciens, genetic transformation

中图分类号:

唐桂容, 刘建雨, 于海龙, 宋春艳, 谭琦, 尚晓冬. 根癌农杆菌介导的金针菇遗传转化研究进展[J]. 生物技术进展, 2024, 14(6): 902-910.

Guirong TANG, Jianyu LIU, Hailong YU, Chunyan SONG, Qi TAN, Xiaodong SHANG. Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens[J]. Current Biotechnology, 2024, 14(6): 902-910.

图/表 2

参考文献 61

1	葛再伟,刘晓斌,赵宽,等.冬菇属的新变种和中国新记录种[J].菌物学报,2015,34(4):589-603.
	GE Z W, LIU X B, ZHAO K, et al.. Species diversity of Flammulina in China: new varieties and a new record[J]. Mycosystema, 2015, 34(4): 589-603.
2	WANG P M, LIU X B, DAI Y C, et al.. Phylogeny and species delimitation of Flammulina: taxonomic status of winter mushroom in East Asia and a new European species identified using an integrated approach[J]. Mycol. Prog., 2018, 17(9): 1013-1030.
3	戴玉成,杨祝良.中国五种重要食用菌学名新注[J].菌物学报,2018,37(12):1572-1577.
	DAI Y C, YANG Z L. Notes on the nomenclature of five important edible fungi in China[J]. Mycosystema, 2018, 37(12): 1572-1577.
4	MAHFUZ S, HE T F, MA J Y, et al. Mushroom stem residue on growth performance, meat quality, antioxidant status and lipid metabolism of broilers[J]. Ital. J. Anim. Sci., 2020, 19(1): 803-812.
5	李晓,李玉.中国工厂化瓶栽白色金针菇竞品分析[J].中国食用菌,2014,33(2):20-24.
	LI X, LI Y. Quality comparison and analysis on white Flammulina velutipes grown with bottle lines in China[J]. Edible Fungi China, 2014, 33(2): 20-24.
6	刘询,刘天海,王波,等.金针菇分子生物学和功能基因研究进展[J].菌物学报,2023,42(1):130-142.
	LIU X, LIU T H, WANG B, et al.. Research progress of molecular biology and functional genes of Flammulina filiformis [J]. Mycosystema, 2023, 42(1): 130-142.
7	MAEHARA T, YOSHIDA M, ITO Y, et al.. Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain[J]. Biosci. Biotechnol. Biochem., 2010, 74(5): 1126-1128.
8	KIM J K, PARK Y J, KONG W S, et al.. Highly efficient electroporation-mediated transformation into edible mushroom Flammulina velutipes [J]. Mycobiology, 2010, 38(4): 331-335.
9	刘建雨, 张美彦, 张丹, 等. 电击法转化金针菇菌丝碎片表达报告基因eGFP及GUS的研究[J]. 食用菌学报, 2018, 25(2): 23-28.
	LIU J Y, ZHANG M Y, ZHANG D, et al. Electroporation of mycelial fragments to express reporter genes eGFP and GUS in Flammulina velutipes [J]. Acta Edulis Fungi, 2018, 25(2):23-28.
10	SHI L, CHEN D, XU C, et al.. Highly-efficient liposome-mediated transformation system for the basidiomycetous fungus Flammulina velutipes [J]. J. Gen. Appl. Microbiol., 2017, 63(3): 179-185.
11	LU Y P, CHEN R L, LONG Y, et al.. A jacalin-related lectin regulated the formation of aerial Mycelium and fruiting body in Flammulina velutipes [J/OL]. Int. J. Mol. Sci., 2016, 17(12): E1884[2024-07-25]. .
12	TAO Y, CHEN R, YAN J, et al.. A hydrophobin gene, Hyd9, plays an important role in the formation of aerial hyphae and primordia in Flammulina filiformis [J]. Gene, 2019, 706: 84-90.
13	沈慧敏,李超,高利,等.原生质体法介导真菌遗传转化的研究进展[J].植物保护,2017,43(2):25-28, 42.
	SHEN H M, LI C, GAO L, et al.. Research progress in transformation of fungi mediated by protoplasts[J]. Plant Prot., 2017, 43(2): 25-28, 42.
14	BOURRAS S, ROUXEL T, MEYER M. Gene transfer: how a plant pathogen hacks the nuclei of plant and nonplant organisms[J]. Phytopathology, 2015, 105(10): 1288-1301.
15	查丽燕,周伟坚,王越,等.根癌农杆菌介导的食用菌遗传转化研究进展[J].食用菌学报,2020,27(1):105-118.
	ZHA L Y, ZHOU W J, WANG Y, et al.. Research progress on Agrobacterium-mediated transformation of edible fungi[J]. Acta Edulis Fungi, 2020, 27(1): 105-118.
16	孙运奇,张津京,冯志勇,等.根癌农杆菌介导的食用菌转化研究[J].食用菌学报,2014,21(1):69-75.
	SUN Y Q, ZHANG J J, FENG Z Y, et al.. Agrobacterium-mediated transformation of edible fungi[J]. Acta Edulis Fungi, 2014, 21(1): 69-75.
17	胡懋,曾杨璇,苗华彪,等.根癌农杆菌介导真菌遗传转化的研究及应用[J].微生物学通报,2021,48(11):4344-4363.
	HU M, ZENG Y X, MIAO H B, et al.. Research and application of Agrobacterium tumefaciens-mediated fungal genetic transformation[J]. Microbiol. China, 2021, 48(11): 4344-4363.
18	刘建雨,徐珍,张丹,等.农杆菌介导法转化金针菇不同受体的效率比较[J].食用菌学报,2015,22(3):7-12.
	LIU J Y, XU Z, ZHANG D, et al.. Efficiency of Flammulina velutipes transformation by Agrobacterium tumefaciens-mediated transformation using different receptors[J]. Acta Edulis Fungi, 2015, 22(3): 7-12.
19	OKAMOTO T, YAMADA M, SEKIYA S, et al.. Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic Mycelium of the cultivated mushroom Flammulina velutipes [J]. Biosci. Biotechnol. Biochem., 2010, 74(11): 2327-2329.
20	CHO J H, LEE S E, CHANG W B, et al.. Agrobacterium-mediated transformation of the winter mushroom, Flammulina velutipes [J]. Mycobiology, 2006, 34(2): 104-107.
21	YAN L, XU R, ZHOU Y, et al.. Effects of medium composition and genetic background on Agrobacterium-mediated transformation efficiency of Lentinula edodes [J/OL]. Genes, 2019, 10(6): E467[2024-07-25]. .
22	乔燕楠,林星雨,高雅,等.根癌农杆菌介导的双孢蘑菇转基因体系的建立[J].食用菌学报,2021,28(1):48-54.
	QIAO Y N, LIN X Y, GAO Y, et al.. Establishment of A high-efficiency Agrobacterium-mediated transformation system in Agaricus bisporus [J]. Acta Edulis Fungi, 2021, 28(1): 48-54.
23	ZHANG J J, SHI L, CHEN H, et al. An efficient Agrobacterium- mediated transformation method for the edible mushroom[J/OL]. Microbiol. Res., 2014, 169(9-10):741-748.
24	LEI M, WU X, ZHANG J, et al.. Establishment of an efficient transformation system for Pleurotus ostreatus [J/OL]. World J. Microbiol. Biotechnol., 2017, 33(12): 214[2024-07-25]. .
25	陈焕,范天宁,白玉东,等. 金针菇中免疫调节蛋白编码基因FIP-fve 的功能[J]. 华中农业大学学报, 2023, 42(2):131-138.
	CHEN H, FAN T Y, BAI Y D, et al.. Native bio-function of FIP-fve towards Flammulina filiformis [J]. Huazhong Agric.Univ., 2023, 42(2):131-138.
26	SEKIYA S, YAMADA M, SHIBATA K, et al.. Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes [J]. J. Biosci. Bioeng., 2013, 115(4): 360-365.
27	LIU Z, DENG B, YUAN H, et al.. Transcription factor FfMYB15 regulates the expression of cellulase gene FfCEL6B during mycelial growth of Flammulina filiformis [J/OL]. Microb. Cell Fact., 2022, 21(1): 216[2024-07-25]. .
28	DU M, XIE Y, WANG M, et al.. Ffga1 protein is essential for regulating vegetative growth, cell wall integrity, and protection against stress in Flammunina filiformis [J/OL]. J. Fungi Basel Switz., 2022, 8(4): 401[2024-07-25]. .
29	LIU Y, MA X, LONG Y, et al.. Effects of β- 1, 6-glucan synthase gene (FfGS6) overexpression on stress response and fruit body development in Flammulina filiformis[J/OL]. Genes, 2022, 13(10): 1753[2024-07-25]. .
30	WU T, HU C, XIE B, et al.. A single transcription factor (PDD1) determines development and yield of winter mushroom (Flammulina velutipes)[J/OL]. Appl. Environ. Microbiol., 2019, 85(24): e01735-19[2025-10-10]. ‍.
31	LI J, SHAO Y, YANG Y, et al.. The chromatin modifier protein FfJMHY plays an important role in regulating the rate of mycelial growth and stipe elongation in Flammulina filiformis [J/OL]. J. Fungi Basel Switz., 2022, 8(5): 477[2024-07-25]. .
32	LYU X, JIANG S, WANG L, et al.. The Fvclp1 gene regulates mycelial growth and fruiting body development in edible mushroom Flammulina velutipes [J]. Arch. Microbiol., 2021, 203(9): 5373-5380.
33	LIU F, CHOU T, WANG W, et al.. Homeodomain 1 genes of the different HD subloci of Flammulina velutipes can activate the HD pathway and are involved in mating, clamp cell formation, and upregulation of FvClp1[J]. J. Agric. Food Chem., 2024, 72(17): 9915-9922.
34	WU T, ZHANG Z, HU C, et al.. A WD40 protein encoding gene Fvcpc2 positively regulates mushroom development and yield in Flammulina velutipes [J/OL]. Front. Microbiol., 2020, 11: 498[2024-07-25]. .
35	施乐乐, PEER A F,郭丽,等.农杆菌介导一个内源HMG-box转录因子fvhom1转化金针菇[J].基因组学与应用生物学,2014,33(6):1268-1274.
	SHI L L, PEER A F, GUO L, et al.. Agrobacterium-mediated transformation of an endogenous HMG-box transcription factor fvhom1 in Flammulina velutipes [J]. Genom. Appl. Biol., 2014, 33(6): 1268-1274.
36	MENG L, LYU X, SHI L, et al.. The transcription factor FvHmg1 negatively regulates fruiting body development in winter mushroom Flammulina velutipes [J/OL]. Gene, 2021, 785: 145618[2024-07-25]. .
37	WU T, HU C, XIE B, et al.. A putative transcription factor LFC1 negatively regulates development and yield of winter mushroom[J]. Appl. Microbiol. Biotechnol., 2020, 104(13): 5827-5844.
38	YAN J, CHEKANOVA J, LIU Y, et al.. Reactive oxygen species distribution involved in stipe gradient elongation in the mushroom Flammulina filiformis [J/OL]. Cells, 2022, 11(12): 1896[2024-07-25]. .
39	BURNS C, LEACH K M, ELLIOTT T J, et al.. Evaluation of Agrobacterium-mediated transformation of agricus bisporus using a range of promoters linked to hygromycin resistance[J]. Mol. Biotechnol., 2006, 32(2): 129-138.
40	CHEN X, STONE M, SCHLAGNHAUFER C, et al.. A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus [J]. Cancers, 2000, 66(10): 4510-4513.
41	KUO C Y, CHOU S Y, HSEU R S, et al.. Heterologous expression of EGFP in enoki mushroom Flammulina velutipes [J]. Bot. Stud., 2010, 51:303-309.
42	AL-SALIHI S A A, SCOTT T A, BAILEY A M, et al.. Improved vectors for Agrobacterium mediated genetic manipulation of Hypholoma spp. and other homobasidiomycetes[J]. J. Microbiol. Meth., 2017, 142: 4-9.
43	BURNS C, GREGORY K E, KIRBY M, et al.. Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns[J]. Fungal Genet. Biol., 2005, 42(3): 191-199.
44	WINANS S C. Two-way chemical signaling in Agrobacterium-plant interactions[J]. PLoS One, 1992, 56(1): 12-31.
45	PCOMBIER J, MELAYAH D, RAFFIER C, et al.. Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum [J]. FEMS Microbiol. Lett., 2003, 220(1): 141-148.
46	SHI L, FANG X, LI M, et al.. Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum [J]. World J. Microbiol. Biotechnol., 2012, 28(1): 283-291.
47	刘建辉,张俊玲,李亮,等. 金针菇tps1基因序列分析及不同温度下tps1、tps2基因定量表达研究[J].食品工业科技, 2015, 36(16): 173-182.
	LIU J H, ZHANG J L, LI L, et al.. Sequence analysis of tps1 and relative expression of tps1 and tps2 under different temperature in Flammulina velutipes [J]. Sci. Technol. Food Ind., 2015, 36(16):173-182.
48	刘建雨,刘建辉,徐珍,等.金针菇异戊烯基焦磷酸异构酶基因的克隆及表达分析[J].食用菌学报,2016,23(3):10-14.
	LIU J Y, LIU J H, XU Z, et al.. Cloning and expression of an isopentenyl diphosphate isomerase gene from Flammulina velutipes [J]. Acta Edulis Fungi, 2016, 23(3): 10-14.
49	刘建雨,李婧霆,陆欢,等.基于全基因组的金针菇光受体基因鉴定及表达分析[J].菌物学报,2023,42(3):759-769.
	LIU J Y, LI J T, LU H, et al.. Genome-wide characterization and expression analysis of the photoreceptors encoded in Flammulina filiformis [J]. Mycosystema, 2023, 42(3): 759-769.
50	许畅,罗兴超,张豪,等.金针菇光受体隐花色素Ffcry基因的鉴定及其表达模式[J].菌物学报,2022,41(6):962-970.
	XU C, LUO X C, ZHANG H, et al.. Identification and expression pattern of photoreceptor cryptochrome Ffcry gene in Flammulina filiformis [J]. Mycosystema, 2022, 41(6): 962-970.
51	YAMADA M, SAKURABA S, SHIBATA K, et al.. Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes [J]. Appl. Microbiol. Biotechnol., 2005, 67(2): 240-246.
52	YUN Y H, KOO J S, KIM S H, et al.. Cloning and expression analysis of phenylalanine ammonia-lyase gene in the Mycelium and fruit body of the edible mushroom Flammulina velutipes [J]. Mycobiology, 2015, 43(3): 327-332.
53	KIM H I, LEE C S, JPARK Y. Further characterization of hydrophobin genes in genome of Flammulina velutipes [J]. Mycoscience, 2016, 57(5): 320-325.
54	HUANG Q H, HAN X, MUKHTAR I, et al. Identification and expression patterns of fvexpl1, an expansin-like protein-encoding gene, suggest an auxiliary role in the stipe morphogenesis of Flammulina velutipes [J]. J. Microbiol. Biotechnol., 2018, 28(4):622-629.
55	陶永新,段静怡,李依宁,等.金针菇L-赖氨酸合成通路基因鉴定及对不同光质的响应表达[J].食用菌学报,2018,25(4):1-8.
	TAO Y X, DUAN J Y, LI Y N, et al.. Identification of genes in Flammulina filiformis L-lysine biosynthesis pathway and their expression in response to light conditions[J]. Acta Edulis Fungi, 2018, 25(4): 1-8.
56	刘建雨,王瑞娟,张丹,等.基于金针菇全基因组的赖氨酸合成途径关键酶分析[J].微生物学通报,2016,43(10):2225-2233.
	LIU J Y, WANG R J, ZHANG D, et al.. Analysis of genes related to lysine biosynthesis based on whole genome of Flammulina velutipes [J]. Microbiol. China, 2016, 43(10): 2225-2233.
57	LIU Q, MENG G, WANG M, et al.. Safe-harbor-targeted CRISPR/Cas9 system and Cmhyd1 overexpression enhances disease resistance in Cordyceps militaris [J]. J. Agric. Food Chem., 2023, 71(41): 15249-15260.
58	王晨.香菇T-DNA插入突变体库构建和色氨酸合酶基因功能分析[D].武汉:华中农业大学,2021.
59	WALTZ E. Gene-edited CRISPR mushroom escapes US regulation[J/OL]. Nature, 2016, 532: 293[2024-07-25]. .
60	刘建雨,刘建辉,张丹,等.农杆菌介导的Cas9基因转化金针菇的研究[J].食用菌学报,2017,24(3):25-29.
	LIU J Y, LIU J H, ZHANG D, et al.. Agrobacterium-mediated gene transformation of Cas9 into Flammulina velutipes [J]. Acta Edulis Fungi, 2017, 24(3): 25-29.
61	林金德,杨雪琴,魏韬,等.金针菇G蛋白偶联受体基因的CRISPR/Cas9基因组编辑载体构建及转化研究[J].菌物学报,2019,38(3):349-361.
	LIN J D, YANG X Q, WEI T, et al.. Construction and transformation of CRISPR/Cas9 genome editing vector of Flammulina filiformis G protein-coupled receptor gene[J]. Mycosystema, 2019, 38(3): 349-361.

基因名	功能	受体材料	根癌农杆菌	质粒	启动子	选择标记	参考文献
FIP-fve	调控菌丝生长	菌丝体	LBA4404	pCAMBIA1300	F. velutipes gpd	Hyg	[25]
Fv-ada	调控菌丝生长	菌块	LBA4404	pFungiway	F. velutipes gpd	Hyg	[26]
FfCEL6B	调控菌丝生长	菌块	GV3101	pCAMBIA1300	GpdII	Hyg	[27]
FfGa1	调控菌丝生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[28]
FfGS6	调控菌丝对外界胁迫与子实体生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[29]
Pdd1	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[30]
FFJMHY	调控菌丝、菌柄生长	菌块	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[31]
Fvclp1	调节菌丝生长和子实体发育	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[32]
FvHd1	调控锁状联合形成	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[33]
FvCPC2	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[34]
Fvhom1	调控菌丝生长	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[35]
Hmg1	调节子实体生长速度	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[36]
LFC1	调节子实体生长	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[37]
FFMnSOD1	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FFMnSOD2	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FfNoxA	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]

基因名	功能	受体材料	根癌农杆菌	质粒	启动子	选择标记	参考文献
FIP-fve	调控菌丝生长	菌丝体	LBA4404	pCAMBIA1300	F. velutipes gpd	Hyg	[25]
Fv-ada	调控菌丝生长	菌块	LBA4404	pFungiway	F. velutipes gpd	Hyg	[26]
FfCEL6B	调控菌丝生长	菌块	GV3101	pCAMBIA1300	GpdII	Hyg	[27]
FfGa1	调控菌丝生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[28]
FfGS6	调控菌丝对外界胁迫与子实体生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[29]
Pdd1	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[30]
FFJMHY	调控菌丝、菌柄生长	菌块	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[31]
Fvclp1	调节菌丝生长和子实体发育	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[32]
FvHd1	调控锁状联合形成	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[33]
FvCPC2	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[34]
Fvhom1	调控菌丝生长	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[35]
Hmg1	调节子实体生长速度	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[36]
LFC1	调节子实体生长	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[37]
FFMnSOD1	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FFMnSOD2	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FfNoxA	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]

[1]	邹凯. 植物毛状根研究及应用[J]. 生物技术进展, 2024, 14(3): 341-348.
[2]	李文辉, 王逸璇, 贠建民, 王彪, 郭更新, 屈玉玲. 马铃薯淀粉渣对金针菇生长特性及产量的影响[J]. 生物技术进展, 2023, 13(3): 435-440.
[3]	薛琴琴,,韩贝贝,吴雪晴,李沛,李莹莹,,吴庆钰. 纳米材料在农作物领域的应用及展望[J]. 生物技术进展, 2020, 10(6): 655-660.
[4]	刘宏，贠建民，毛永强，何田田，毕阳. 两种精油熏蒸处理对金针菇贮藏品质的影响[J]. 生物技术进展, 2020, 10(2): 190-197.
[5]	费凡,冯伟伟,茆广华,张伟杰,丁阳阳,赵婷,吴向阳. 金针菇菌渣和醋渣固态发酵的工艺研究[J]. 生物技术进展, 2019, 9(4): 422-429.
[6]	胡海英,吴晓玲. 银柴胡离体培养与毛状根诱导技术初步研究[J]. 生物技术进展, 2015, 5(6): 436-440.
[7]	刘大普, 杨凤萍, 张晓东. 麦类作物小孢子培养与遗传转化研究进展[J]. 生物技术进展, 2012, 2(6): 385-390.
[8]	姚冉,石美丽,潘沈元,沈桂芳,张志芳. 农杆菌介导的植物遗传转化研究进展[J]. 生物技术进展, 2011, 1(4): 260-265.
[9]	史庆玲. 玉米转基因方法研究进展[J]. 生物技术进展, 2011, 1(3): 178-183.
[10]	刘亚,李敬娜,任雯,李翔. 植物遗传转化表达载体研究进展[J]. 生物技术进展, 2011, 1(1): 14-20.

根癌农杆菌介导的金针菇遗传转化研究进展

Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 61

相关文章 10

编辑推荐

Metrics

本文评价